13-Acetoxysarcocrassolide was purified after extracting it from the cultured Formosa soft coral S. crassocaule, according to the method described by others (Duh et al. 2000 (link)). Dimethyl sulfoxide (DMSO), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), Z-DEVD-FMK (caspase-3 inhibitor), Z-VAD-FMK (a cell-permeable pan-caspase inhibitor), a protease inhibitor cocktail, and rabbit anti-human β-actin antibodies were purchased from Sigma (St. Louis, MO, USA). Antibodies against pro-caspase 9, cleaved-caspase 9, pro-caspase 3, cleaved-caspase 3, cleaved-PARP-1, Bax, Bad, Bcl-xL, Mcl-1, p-Bad, PARP-1, Bcl-2, PI3K, p-PI3K, AKT, p-AKT, mTOR, p-mTOR, p70S6K, p-p70S6K, 4EBP1, p-4EBP1, p-S6, p-eIF4B, and p-eIF4E, and goat anti-rabbit and horseradish peroxidase-conjugated immunoglobulin G were obtained from Cell Signalling Technology (Danvers, MA, USA). Dulbecco’s modified Eagle’s medium (DMEM) and foetal bovine serum (FBS) were obtained from Biowest (Nuaillé, France).
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2 h tetrazolium bromide mtt dimethyl sulfoxide dmso
Manufactured by Merck Group
Sourced in United States
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) is a chemical compound used in cell proliferation and viability assays. Dimethyl sulfoxide (DMSO) is a polar aprotic solvent that is commonly used as a solvent for MTT and other biological samples.
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Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
P eif4b, by Cell Signaling Technology (1 mentions)
Rabbit anti human β actin antibody, by Merck Group (1 mentions)
Z devd fmk, by Merck Group (1 mentions)
P p70s6k, by Cell Signaling Technology (1 mentions)
P eif4e, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle s medium dmem, by Biowest (1 mentions)
Z vad fmk, by Merck Group (1 mentions)
4e bp1, by Cell Signaling Technology (1 mentions)
P mtor, by Cell Signaling Technology (1 mentions)
P 4ebp1, by Cell Signaling Technology (1 mentions)
P70s6k, by Cell Signaling Technology (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Foetal bovine serum (fbs), by Biowest (1 mentions)
Ketamine, by Merck Group (1 mentions)
Xylazine, by Merck Group (1 mentions)
Trizol solution, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Kim 1, by Santa Cruz Biotechnology (1 mentions)
8 protocols using 3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2 h tetrazolium bromide mtt dimethyl sulfoxide dmso
1
Purification of 13-Acetoxysarcocrassolide from Soft Coral
2
In Vitro Evaluation of Atorvastatin's Cytoprotective Effects
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Minimum essential medium eagle-alpha modification (α-MEM), dulbecco’s modified eagle medium (DMEM), fetal bovine serum (FBS) and penicillin-streptomycin were purchased from Gibco Life Technologies (Grand Island, NE, USA). Atorvastatin ([R-(R*, R*)-2-(4-Fluorophenyl)-ß, d-dihydroxy-5-(1-methylethyl)-3-Phenyl-4-(Phenylamino)-Carbonyl]-1-H-Pyr- role-1-heptanoic acid), dimethyl sulfoxide (DMSO), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), trizol solution and 2 × Real Rime SYBER GREEN master mix, phosphate-buffered saline (PBS), ketamine and xylazine were purchased from Sigma-Aldrich (St. Louis, MO). Terminal deoxy- nucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) Kit was obtained from (Roche, Mannheim, Germany).
3
Alisol A 24-acetate Induces Autophagy in Renal Cells
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Trypsin was provided by KeyGEN Biotech Co., Ltd (Nanjing, China). Dulbecco’s modified eagle medium (DMEM)-F12 (1:1) medium with high-glucose and fetal bovine serum (FBS) were purchased from Gibco/BRL (Grand Island, New York, NY, USA). 3-4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2-H-tetrazolium bromide (MTT), dimethylsulfoxide (DMSO), and 3-methyl adenine (3-MA) were provided by Sigma Chemical (St. Louis, MO, USA). Alisol A 24-acetate (24A) and Alisol B 23-acetate (23B; purity ≥95%) were purchased from Tianjin Evans Science and Technology Co., Ltd (Tianjin, China). Primary and second antibodies against LC3, Beclin-1, Bcl-2, Bcl-xl, Clusterin, Kim-1, TFF-3 and β-actin were provided by Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Hematoxylin-eosin staining solution was offered by Shanghai Yuanmu Biotechnology Co., Ltd (Shanghai, China). EliVision plus and DAB kits were offered by MXB Biological Technology Co., Ltd (Fuzhou, Fujian, China). Monopotassium phosphate, disodium hydrogen phosphate, sodium chloride, and potassium chloride were obtained from Nanjing Nanao Science and Technology Co., Ltd (Nanjing, China).
4
Fucus-Mediated Gold Nanoparticle Synthesis
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Fu extracted from Fucus vesiculosus was obtained from Sigma (Sigma, St. Louis, Mo, U.S.A.). Chloroauric acid trihydrate (HAuCl4∙3H2O, > 99.9%) and doxorubicin hydrochloride (Dox·HCl) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Dulbecco’s modified eagle’s medium/F12 (DMEM-F12) was obtained from Cellgro (Mediatech, Massachusetts, USA). Dulbecco’s modified eagle’s medium (DMEM), trypsin–ethylenediaminetetraacetic acid (trypsin-EDTA), antibiotics, fetal bovine serum (FBS), and phosphate-buffered saline (PBS) were purchased from Gibco BRL, Life Technologies (Grand Island, NY, USA). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), Hoechst 33342, and propidium iodide (PI) were obtained from Sigma-Aldrich. Double-distilled water was used for all aqueous solutions in the experiments. All chemicals were used directly as received without further purification.
5
DTNB and NADPH Cytotoxicity Assay
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5,5’-Dithiobis-(2-nitrobenzoic acid) (DTNB) and β-nicotinamide adenine dinucleotide 2’-phosphate reduced tetrasodium salt (NADPH) were purchased from Roche (Basel, Switzerland). RPMI 1640 and fetal bovine serum were from Gibco (Invitrogen Corporation, Carlsbad, USA). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO) and PZQ were sourced from Sigma-Aldrich Inc. (Saint Louis, USA). The Nitric Oxide Assay Kit and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (carboxy-PTIO) came from Beyotime Biotechnology Corporation (Shanghai, China). Ethylenediaminetetraacetic acid (EDTA) and 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) were purchased from Sangon Biotech Co. Ltd. (Shanghai, China), and artemisinin (AS) was purchased from Zelang Medical Technology Co. Ltd. (Nanjing, China).
6
ESC Extraction and Component Analysis
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Process of ESC (Extract of Stellera Chamaejasme L.) and determination of part components of ESC was provided in another paper [11 (link)]. Trypsin-ethylene-diaminetetraacetic acid (EDTA) and DMEM medium were purchased from Gibco (Grand Island, NY, USA);3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO) and SB431542 were provided by Sigma Chemical Co. (St. Louis, MO, USA); TGF-β was from R&D Systems (Miniieapolis, MN, USA); E-cadherin, Vimentin and β-actin primary monoclonal antibody were purchased by Abcam Ltd (Cambridgem MA, USA); Matrigel was from BD Biosciences (Los Angeles, CA, USA); Crystal violet was from Beijing Solarbio Science and Technology Co., Ltd; Smad and p-Smad primary monoclonal antibody were from Cell Signal Technology, Inc (Beverly, MA, USA).
7
Cannabinoid Receptor Modulation Effects
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HT22 cells were obtained from the Fourth Military Medical University. Sprague-Dawley (SD) rats were purchased from the Laboratory Animal Center of Sun Yat-sen University. The CB1 cannabinoid receptor agonist ACEA, CB1 receptor antagonist AM251 (can penetrate the cell membrane), cell membrane CB1 receptor antagonist hemopressin (Hemo), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), DMEM cell culture medium and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
8
Bioactive Compounds from Astragalus Roots
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We purchased dried roots of A. membranaceus of superior quality from Qi Jing Ltd. (Beijing, China) and H22 cells (murine hepatocarcinoma cells) from the Shanghai Institute of Biological Sciences at the Chinese Academy of Sciences (Shanghai, China). We purchased 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), DEAE-cellulose 52, Sephadex G-100, and galacturonic acid from Sigma (St. Louis, MO, USA). We purchased bovine serum albumin (BSA), T-series dextran, standard monosaccharides, glucuronic acid, and 5-Fu from Solarbio Co. Ltd. (Shanghai, China). All of the chemicals and agents were of analytical grade.
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