Total proteins were extracted from isolated EVs by lysing samples with ice-cold RIPA buffer supplemented with SIGMAFAST™ Protease Inhibitors and Phosphatase Inhibitor Cocktail 3 and 2 (all from Sigma). Proteins were boiled with Laemmli SDS sample buffer 6X (VWR International), separated on 4–20% MiniPROTEAN®TGX™ Precast Gel, and transferred onto a PVDF membrane with a semi-dry transfer system (all from Bio-Rad Europe, Basel, Switzerland). Membranes were incubated with ALIX (Abcam ab186429; 1:1000), TSG101 (Abcam ab125011, 1:1000), and Syntenin-1 (Abcam ab19903; 1:1000) and Apolipoprotein A1 (APOA1) (Invitrogen 701239, 1:500) primary antibodies. Secondary antibodies IRDye® 680RD or 800CW goat anti-mouse or goat anti-rabbit (LI-COR Biosciences) were used for detection. The infrared signal was detected using Odyssey CLx Detection System (LI-COR Biosciences).
800cw goat anti mouse or goat anti rabbit
Manufactured by LI COR
Sourced in United States
The 800CW goat anti-mouse or goat anti-rabbit is a secondary antibody product used for detection in Western blotting and other immunoassay applications. It is a conjugate of a goat-derived polyclonal antibody that specifically binds to mouse or rabbit primary antibodies, and the near-infrared dye IR800CW. This allows for sensitive and quantitative detection of target proteins on Western blots using near-infrared fluorescence technology.
Automatically generated - may contain errors
Lab products found in correlation
Odyssey clx detection system, by LI COR (2 mentions)
Irdye 680rd, by LI COR (2 mentions)
Mini protean tgx precast gel, by Bio-Rad (1 mentions)
Sigmafast protease inhibitors and phosphatase inhibitor cocktail 3 and 2, by Merck Group (1 mentions)
Laemmli sds sample buffer 6x, by Avantor (1 mentions)
Anti grp94, by Abcam (1 mentions)
Anti periostin, by Santa Cruz Biotechnology (1 mentions)
Anti tsg101, by Abcam (1 mentions)
Anti gapdh, by Abcam (1 mentions)
2 protocols using 800cw goat anti mouse or goat anti rabbit
1
Extracellular Vesicle Protein Profiling
2
Exosomal Protein Analysis by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total proteins were extracted by lyzing exosomes or cells in the presence of protease inhibitors. Proteins were separated on gel and transferred onto a PVDF membrane. Anti-Periostin (Santa Cruz, United States), anti-TSG101, anti-GRP94, and anti-GAPDH (Abcam, United Kingdom) were used as primary antibodies; IRDye® 680RD or 800CW goat anti-mouse or goat anti-rabbit (LI-COR Biosciences, United States) as secondary antibodies. The infrared signal was detected using the Odyssey CLx Detection System (LI-COR Biosciences).
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