Alexa 555 conjugated goat anti rat igg

Manufactured by Thermo Fisher Scientific

Alexa 555-conjugated goat anti-rat IgG is a secondary antibody that binds to rat immunoglobulin G (IgG). The antibody is conjugated to the Alexa Fluor 555 dye, which enables fluorescent detection and visualization of target proteins in various applications, such as immunohistochemistry, flow cytometry, and Western blotting.

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Lab products found in correlation

Mouse anti human cd31, by Agilent Technologies (1 mentions) Rabbit anti human vwf, by Agilent Technologies (1 mentions) Axiovision v4, by Zeiss (1 mentions) Axiovert 200 microscope, by Zeiss (1 mentions) Rat anti mouse cd31, by BD (1 mentions) Lsm 710 confocal laser scanning microscope, by Zeiss (1 mentions) Anti b220 efluor 450, by Thermo Fisher Scientific (1 mentions) Zen software, by Zeiss (1 mentions)

2 protocols using alexa 555 conjugated goat anti rat igg

Immunofluorescence Staining of Vascular Markers

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Immunostaining was performed using the following primary antibodies: rabbit anti-human VWF (1:150; Dako), mouse anti-Human TSP (1:75, Laboratory Vision/ Neomarkers) rat anti-mouse CD31 (1:50; BD Biosciences), rat anti-mouse GPIbα/CD42 (1:100; EMFRET Analytics), mouse anti-human CD31 (1:50; Dako), and rabbit anti-mouse vascular endothelial growth factor-A (VEGF-A) (1:150; Santa Cruz). Following secondary antibodies were used: fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG (1:200; BD Pharmingen), Alexa 555-conjugated goat anti-rat IgG (1:200; Invitrogen). Nuclei were counterstained with DAPI (1:1000). Fluorescence images were acquired with a Zeiss Axiovert 200 microscope and were analyzed using AxioVision v4.8 (Zeiss) and ImageJ v1.47c.

Robador J.R., Feinauer M.J., Schneider S.W., Mayer F.T., Gorzelanny C., Sacharow A., Liu X., Berghoff A., Brehm M.A., Hirsch D., Stadler J., Vidal-y-Si S., Wladykowski E., Asong M., Nowak K., Seiz-Rosenhagen M., Umansky V., Mess C., Pantel K., Winkler F, & Bauer A.T. (2021). Involvement of platelet-derived VWF in metastatic growth of melanoma in the brain. Neuro-oncology Advances, 3(1), vdab175.

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Immunofluorescence Staining of Mouse Spleen

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Spleens were snap-frozen in O.C.T. medium (Tissue-Tek; Sakura, Alphen aan den Rijn, The Netherlands). Cryosections (10 μm) were fixed in ice cold acetone for 10 minutes, air-dried, and blocked in 1% BSA plus 20% FBS in PBS before staining with Abs at room temperature. Cryosections were incubated with rat anti-mouse Siglec-1 (clone SER-4; provided by Dr. J. den Haan, VUMC, Amsterdam, The Netherlands) followed by Alexa555-conjugated goat anti-rat IgG (Invitrogen), anti-SIGN-R1 Alexa647 (clone 22D1; Dr. J. den Haan) and anti-B220 eFluor450 (eBioscience). Images were acquired using a Zeiss LSM 710 confocal laser scanning microscope with Zen software (Carl Zeiss Microimaging, Jena, Germany).

Haeberlein S., Obieglo K., Ozir-Fazalalikhan A., Chayé M.A., Veninga H., van der Vlugt L.E., Voskamp A., Boon L., den Haan J.M., Westerhof L.B., Wilbers R.H., Schots A., Schramm G., Hokke C.H, & Smits H.H. (2017). Schistosome egg antigens, including the glycoprotein IPSE/alpha-1, trigger the development of regulatory B cells. PLoS Pathogens, 13(7), e1006539.

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