Primer and probe sets fam vic labelled

Cells were prepared as described above. Total cellular RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA). Real-time RT-PCR was performed using RNA (20 ng) as the template, qScript cDNA SuperMix for the reverse transcription step, and PerfeCTa qPCR FastMix, UNG, and ROX for PCR (Quanta Biosciences, Gaithersburg, MD, USA). Primer and probe sets (FAM/VIC-labelled) were purchased from Applied Biosystems (Waltham, MA, USA). The results were normalised based on the values obtained for GAPDH as detected using TaqMan GAPDH control reagents (Applied Biosystems). Real-time qPCR was performed with samples in duplicate using the ABI 7700 Sequence Detection System (Applied Biosystems). For cells from each donor, relative expression levels based on 2^−ΔΔCT values are shown as percentages relative to values obtained for the subset with the highest expression.

Cells were prepared as described above. Total cellular RNA was isolated using TRIzol reagent (Invitrogen). Real-time RT-PCR was performed using RNA (20 ng) as the template, qScript cDNA SuperMix for the reverse transcription step, and PerfeCTa qPCR FastMix, UNG, and ROX for PCR (Quanta Biosciences, Gaithersburg, MD, USA). Primer and probe sets (FAM/VIC-labelled) were purchased from Applied Biosystems (Waltham, MA, USA). The results were normalized based on the values obtained for GAPDH as detected using TaqMan GAPDH control reagents (Applied Biosystems). Real-time qPCR was performed with samples in duplicate using the ABI 7700 Sequence Detection System (Applied Biosystems). For cells from each donor, relative expression levels based on 2^−ΔΔCT values are shown as percentages relative to values obtained for the subset with the highest expression.