To express channelrhodopsin2 (ChR2) in CA1 pyramidal cells for optogenetic generation of gamma oscillations in the CA1 subregion, CaMKII-Cre mice (the Jackson Laboratory stock # 005359) were crossed with floxed ChR2 mice (the Jackson Laboratory stock # 012569) to produce mice expressing ChR2 in CaMKII-expressing cells (referred to as CaMKII-ChR2 mice in this study). To efficiently target PVBCs for whole-cell patch-clamp recordings, we crossed Ai9 Cre reporter mice (the Jackson Laboratory stock # 007905) with PV-Cre mice (the Jackson Laboratory stock # 008069) to produce mice expressing tdTomato in PV + cells (referred to as PV-TOM mice in this study) as previously described (Kang et al., 2018 (link)). Both genders of 4 to 8-week-old CaMKII-ChR2 mice, PV-TOM mice, and C57BL/6 mice (The Jackson Laboratory stock # 000664) were used in the current study.
Ai9 cre reporter mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
The Ai9 Cre reporter mice are a genetically modified mouse line that express the Cre recombinase enzyme under the control of the Rosa26 promoter. This allows for the conditional expression of a tdTomato fluorescent protein upon Cre-mediated recombination.
Automatically generated - may contain errors
Lab products found in correlation
Pv cre mice, by Jackson ImmunoResearch (2 mentions)
Camkii chr2 mice, by Jackson ImmunoResearch (1 mentions)
Camkii cre mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
Wild type c57bl 6j male mice, by Jackson ImmunoResearch (1 mentions)
Npy gfp mice, by Jackson ImmunoResearch (1 mentions)
Wild type c57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
Nih 3 nude mice, by Charles River Laboratories (1 mentions)
Twist2 cre, by Jackson ImmunoResearch (1 mentions)
6 protocols using ai9 cre reporter mice
1
Optogenetic Induction of Gamma Oscillations in CA1
2
Genetically Modified Mice for Cartilage Research
3
Transgenic Mouse Models for Vision Research
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The CD1, Ai9 Cre reporter mice, and Pde6βrd1/rd1 mice were purchased from the Jackson Laboratory. The M-opsin-Cre mice39 (link) (cone-specific Cre line) and the rhodopsin knockout (Rho−/−) mice40 (link) were provided by Yun Z. Le (University of Oklahoma Health Sciences Center) and Janis Lem (Tufts University, Boston), respectively. Ai9+/−_MCre+ mice were generated by crossing MCre+ mice with the Ai9 Cre reporter mice. Pde6βrd1/+_Ai9+/− mice were generated by crossing rd1 mice and Ai9 mice. Heterozygotes were mated to produce Pde6βrd1/rd1/+_Ai9+/− mice.
All animals were maintained at UMASS Medical School under a 12 hour/12 hour light/dark cycle with unrestricted access to food and water. Lighting conditions were kept constant in all cages, with illumination ranging between 10 and 15 lux. All experiments involving mice were conducted in compliance with the Association for Research in Vision and Ophthalmology statement for the use of animals in ophthalmic and vision research. All procedures were approved by Institutional Animal Care and Use Committee of UMASS Medical School.
All animals were maintained at UMASS Medical School under a 12 hour/12 hour light/dark cycle with unrestricted access to food and water. Lighting conditions were kept constant in all cages, with illumination ranging between 10 and 15 lux. All experiments involving mice were conducted in compliance with the Association for Research in Vision and Ophthalmology statement for the use of animals in ophthalmic and vision research. All procedures were approved by Institutional Animal Care and Use Committee of UMASS Medical School.
4
Patch-Clamp Recordings of Interneurons
5
Genetically Modified Mouse Lines for Research
6
Investigating Glut1 Deficiency in Osteoarthritis
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All animal studies were performed in accordance with approval of the Committees on Animal Resources in Washington University in St Louis. Glut1f/f mice were generated as previously described.51 (link)Ai9 Cre reporter mice were purchased from the Jackson Laboratory.52 (link)Agc1CreERT2 mice53 (link) were generous gifts from Dr. Benoit de Crombrugghe (Department of Genetics, University of Texas MD Anderson Cancer Center, Houston, TX). Agc1CreERT2;Glut1f/f (Glut1Agc1ER; Glut1 LOF) mice, Agc1CreERT2;Rosa-Ai9f/+ (Rosa-Ai9Agc1ER) mice and Cre-negative littermates (Glut1f/f and Rosa-Ai9f/+) were viable and produced Mendelian ratios. Tamoxifen was administered daily at a dose of 1 mg per 10 g body weight for 5 consecutive days via intraperitoneal injection to 1-month-old Glut1Agc1ER mice Rosa-Ai9Agc1ER mice, and Cre-negative controls to remove Glut1 alleles or induce Ai9 expression. To determine whether Glut1 LOF modifies the disease progression of injury-induced OA, meniscal ligament injuries (MLIs) were created unilaterally in the knee joints as previously described54 (link) in Glut1Agc1ER mice and their littermates following tamoxifen induction at 1 month. Ethyl-3,4-dihydroxybenzoic acid (DHB; Sigma-Aldrich; #E24859) was administered intraperitoneally to 1-month-old control and Glut1 LOF mice every other day at a dose of 40 mg·kg−1 body weight for 2 months.
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