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2 protocols using glycylglycine

1

Antioxidant Enzyme Activity Assay

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Dichloromethane, ethyl acetate, hexane, sodium bicinchoninate, 7, 12-dimethylbenz(α)anthracene, 5-5-dithiobisnitrobenzoic acid (DTNB), sodium dithionate, L-γ-glutamyl-4-nitroanilide, H2O2, and malondialdehyde (MDA) were purchased from Sigma-aldrich, Bangalore, India. Xylenol orange, ammonium ferrous sulfate, cyclohexane, 2, 6-dichlorophenol-indophenol (DCPIP), reduced nicotinamide adenine dinucleotide (NADH), reduced nicotinamide adenine dinucleotide phosphate (NADPH), bovine serum albumin (BSA), potassium ferricyanide, 1-chloro-2,4-dinitrobenzene (CDNB), glycylglycine, reduced glutathione (GSH), oxidized glutathione (GSSG), ascorbic acid, guaiacol, Triton-X, nitroblue tetrazolium chloride (NBT), hydro-xylamine hydrochloride, and pyruvate were purchased from Himedia Laboratories, Mumbai, India. DMBA (7, 12- dimethylbenz(α)anthracene) was obtained from Sigma-aldrich, Bangalore, India. Autopack kit for analyzing bilirubin was purchased from Beacon Diagnostics, Gujarat, India, while alkaline phosphatase, serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase autopack kits were purchased from Delta Labs, Sindhudurg, India. Rest of the chemicals used in the present study were of analytical grade.
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2

Comprehensive Biochemical Protocol Compendium

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DMBA (7, 12- dimethylbenz(α)anthracene), Indole 3 carbinol (I3C), 5-5′dithiobisnitrobenzoic acid (DTNB) sodium dithionate and malondialdehyde were obtained from Sigma-aldrich, Banglore, India. Xylenol orange, ammonium ferrous sulfate, cyclohexane, 2,6-dichlorophenol-indophenol (DCPIP), reduced nicotinamide adenine dinucleotide (NADH), reduced nicotinamide adenine dinucleotide phosphate (NADPH), bovine serum albumin (BSA), potassium ferricyanide, 1-chloro-2,4-dinitrobenzene (CDNB), glycylglycine, reduced glutathione (GSH), oxidized glutathione (GSSG), ascorbic acid, guiacol, triton X, nitroblue tetrazolium chloride (NBT), hydroxylamine hydrochloride, pyruvate were obtained from Himedia Laboratories, Mumbai, India. All other reagents used in the present study were of analytical grade.
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