Culture, morphophysiology and biochemical characters of the microbial isolates were studied following standard methods (Ventosa et al., 1998 (link)). The isolates were checked for colony characters, motility, shape/size of the organism, and Gram and endospore staining. Biochemical tests, hydrolysis to carbohydrates and its metabolism as well as tolerance to NaCl were performed. Colony characters of isolates were observed on HiCrome Bacillus agar, rapid HiEnterococci agar, rapid HiColiform agar, Enterococcus confirmatory agar, Kligler iron agar, Chapman stone agar, Pikovskaya's agar, KG agar, Pseudomonas isolation agar, Hifluoro Pseudomonas agar base phenylalanine agar, triple sugar-iron agar, coagulase mannitol agar and HiCrome staph agar base for characterization of the bacteria (HiMedia Pvt Ltd, Mumbai).
Hicrome staph agar
Manufactured by HiMedia
Sourced in India
HiCrome staph agar is a selective and differential culture medium used for the isolation and identification of Staphylococcus species from clinical and non-clinical samples. It contains chromogenic substrates that allow the differentiation of Staphylococcus aureus from other Staphylococcus species based on colony color.
Automatically generated - may contain errors
Lab products found in correlation
Triple sugar iron agar, by HiMedia (1 mentions)
Hicrome bacillus agar, by HiMedia (1 mentions)
Pseudomonas isolation agar, by HiMedia (1 mentions)
Cetrimide agar, by HiMedia (1 mentions)
Wilkins chalgren anaerobic agar, by HiMedia (1 mentions)
Reinforced clostridial agar, by HiMedia (1 mentions)
Lactobacillus mrs agar, by HiMedia (1 mentions)
2 protocols using hicrome staph agar
1
Comprehensive Microbial Characterization Protocol
2
Cecal Microbiome Enumeration Protocol
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The ceca with contents were stored at 4°C for determination of cecal microbiota. The cecal digesta was processed within 24 h (5 (link)). Each cecal digesta homogenate was serially diluted from 10-1 to 10-8. Coliforms were cultured using HiCrome E. coli HiVeg Agar (Product code: MV1295; Hi-Media Laboratories, Mumbai, India), anaerobic bacteria were cultured using Wilkins Chalgren Anaerobic Agar (Product code: M832; Hi-Media Laboratories), Staphylococcus aureus was cultured using HiCrome Staph Agar (Product code: M1837; Hi-Media Laboratories), Lactobacillus spp. were cultured using Lactobacillus MRS Agar (Product code: M641; Hi-Media Laboratories), Pseudomonas aeruginosa was cultured using Cetrimide Agar (Product code: M024; Hi-Media Laboratories), and the Clostridium were cultured in Reinforced Clostridial Agar (Product code: M154; Hi-Media Laboratories). Diluted digesta samples were streaked onto the agar plates and incubated at 37°C for 48 h, while the clostridia plates were incubated anaerobically at similar temperature. Visible colonies were enumerated using a colony counter and the results were expressed as log10 CFU/g of cecal digesta.
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