Tgn46 antibody

Mouse monoclonal Flag (M2) antibody was obtained from Sigma-Aldrich. Rabbit polyclonal GFP (A6455), AF647-conjugated Phalloidin, transferrin, and anti–sheep, and Alexa Fluor 488–conjugated anti–mouse and anti–rabbit were purchased from Invitrogen. Chicken polyclonal Rab13 and rabbit monoclonal LAMP-1 antibodies were obtained from Abcam. Rat monoclonal Hsc70 (1B5) antibody was obtained from Enzo Life Sciences. Rabbit polyclonal TIP47 antibody was purchased from Novus Biologicals. Sheep polyclonal TGN46 antibody was obtained from AbD Serotec. HRP-conjugated anti–mouse and anti–rabbit were obtained from Jackson ImmunoResearch Laboratories, Inc.

HET1A squamous oesophageal epithelial cells (ATCC, Rockville, MD, USA), QH Barrett's metaplastic cells and GO Barrett's dysplastic cells (also designated CP-A and CP-C, respectively, kindly provided by Professor Rabinovich, the University of Washington) were cultured in bronchial epithelial cell basal medium with supplements (Lonza, Basel, Switzerland). For the QH and GO cell lines, medium was further supplemented with 5%(v/v) foetal calf serum (Gibco-BRL, Grand Island, NY, USA). SKGT4 oesophageal adenocarcinoma cells (ATCC) were cultured in RPMI with 10% (v/v) foetal calf serum. GM130, β-actin, M1, M2 antibodies and all other chemicals were obtained from Sigma-Aldrich Chemical Company (St Louis, MO, USA). TGN46 antibody was obtained from AbD Serotec (Oxford, UK). GOLPH2 antibody was obtained from Abnova (Abnova Corp,Taipei, Taiwan). Horseradish peroxidase-conjugated secondary antibodies, AlexaFluor-conjugated secondary antibodies and Hoechst were obtained from Invitrogen (Carlsbad, CA, USA).

MMP-2 and Rab11 antibodies were purchased from Abcam (rabbit ab92536 and rabbit ab3612, respectively). NUCB1, β-actin, and monoclonal anti-FLAG M2 peroxidase antibodies were obtained from Sigma-Aldrich (rabbit HPA008176 and mouse A5441 and A8592, respectively); ERGIC53 was acquired from ENZO Life Sciences (mouse ENZ-ABS300); GM130 antibody and β-integrin were purchased from BD Bioscience (mouse 610822 and mouse 610467, respectively); Rab5, Rab7, and streptavidin-HRP antibodies were from Cell Signaling (rabbit C8B1, D95F2, and 3999S, respectively); and TGN46 antibody was obtained from AbD Serotec (sheep AHP500G). MMP-14 antibody was purchased from Millipore (mouse MAB3328), and GFP antibody (rabbit sc8334) and HRP-coupled secondary antibodies (anti-rabbit IgG, anti-mouse IgG, and anti-sheep IgG) were purchased from Santa Cruz Biotechnology. Sec16A antibody (KIAA0310 polyclonal rabbit) was purchased from Biomol (now Thermo Fisher Scientific, A300-648A-M). Anti-rabbit HRP antibody used with primary macrophages was purchased from Cell Signaling (7074). The Alexa Fluor secondary antibodies used for immunofluorescence (488, 594, 633, and phalloidin-Alexa Fluor 488, different species) were purchased from Thermo Fisher Scientific.