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Dpx mounting media

Manufactured by Abcam
Sourced in United States, United Kingdom

DPX mounting media is a xylene-based mounting solution used to permanently mount microscope slides. It provides a transparent, non-fluorescent, and durable mounting environment for microscopic specimens.

Automatically generated - may contain errors

2 protocols using dpx mounting media

1

Potassium oxonate-induced hyperuricemia study

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Potassium oxonate (catalog# 156124), carvacrol (catalog# W224511) were purchased from Sigma-Aldrich (St. Louis, MO, USA), while allopurinol was obtained from Pharmedic Laboratories (Pvt.) Ltd. (Lahore, Pakistan). The primary antibodies TNF-α (SC-52B83) and nuclear factor-kappa B (p-NF-κB) (SC-271908) and other immunohistochemistry-related consumables, including the Elite ABC kit (SC-2018) and 3,3-diaminobenzidine (DAB; SC-216567), were procured from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The biotinylated secondary antibody (ab-6789) and DPX mounting media were purchased from Abcam (Cambridge, MA, USA). The enzyme-linked immunosorbent assay (ELISA) kit for rat NLPR3 (CAS# A5652) was purchased from Shanghai MLBIO Biotechnology Co., Ltd. (Shanghai, China), while the ELISA kit for rat TNF-α (Catalog# E-EL-R0019) was obtained from Elabscience (Houston, TX, USA). The kits for uric acid, blood urea nitrogen, creatinine, and C-reactive protein (CRP) were purchased from Beckman Coulter (Brea, CA, USA). All other consumables including formaldehyde, hydrogen peroxide (H2O2), reduced glutathione (GSH), glutathione S-transferase (GST), catalase, trichloroacetic acid, 1-chloro-2,4-dinitrobenzene (CDNB), ethanol, xylene, and hematoxylin and eosin (H&E) stains were purchased from (Sigma-Aldrich).
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2

Pharmacological Evaluation of Silymarin and Paracetamol

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The pharmaceutical drugs (silymarin and N-acetyl para aminophenol (paracetamol CAS: 103-90-2, C8H9NO2: APAP) of HPLC grade (99%) were supplied by a local pharmaceutical manufacturer and used as raw material. All the antibodies were procured from Santa Cruz Biotechnology, United States, or Abcam, United Kingdom. Phosphate-buffered saline (PBS) tablets were used for all morphological analyses or fresh buffers were prepared for each use. The details and corresponding catalog numbers of the primary antibodies are HO-1 (SC-13691), TRX (SC-20146), Nrf2 (SC-722), COX-2 (SC-514489), p-JNK (SC-6254), TNF-α (SC-52B83), and p-NFκB (SC-271908). Other immunohistochemistry-related consumables, such as AB and C Elite kit (two vials-SC-2018) and 3,3-diaminobenzidine (DAB) (SC-216567), were also provided by Santa Cruz Biotechnology, United States. The biotin secondary antibody (ab-6789) and DPX mounting media were purchased from Abcam United Kingdom. The p-NFκB enzyme-linked immunosorbent assay (ELISA) kit (Cat # SU-B28069) and Nrf2 kit (cat. no. SU-B30429) were purchased from Shanghai Yuchun Biotechnology, China. HO-1 (cat. No. E-EL-R0488), and TNF-α (E-EL-R0019) ELISA kits were purchased from Elabscience.
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