Leaf and root samples were simultaneously harvested on days 20, 28, and 36 after treatment. The plant dry weight was measured after oven drying at 80 °C for 72 h. The leaf area was measured using a hand-held leaf area meter (AM-350, ADC Bioscientific Ltd, Herts, England). The SPAD value of the cucumber leaves were measured using a SPAD-502 (Konica Minolta, Tokyo, Japan)
Am 350
Manufactured by ADC BioScientific
Sourced in United Kingdom
The AM 350 is a laboratory instrument designed for the measurement of soil and plant moisture content. It utilizes a precision sensor to accurately determine the water content of samples, providing reliable data for a variety of agricultural and scientific applications.
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Lab products found in correlation
5 protocols using am 350
1
Precise Leaf and Root Measurements
2
Measuring Plant Growth Parameters
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Plant height (PH) was measured with a scale. Plant fresh and dry weights were weighed by using an electronic balance (Verbal 100 Super, Varanasi, Balance Works, Varanasi, India). After recording fresh weight, samples were oven-dried for dehydration at 70°C for 24 h and then weighed. The number of leaves per pot was counted manually. Leaf area was determined using a leaf area meter (AM 350, ADC Bioscientific, Hoddesdon, Herts, United Kingdom).
3
Plant Growth Measurements under Salinity
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Plant height and phyllode area were measured at days 90th and 150th of salt treatments using measuring scale and Area Meter (AM 350, ADC BioScientific Ltd), respectively. For determining biomasses of phyllodes and roots, plants were carefully uprooted at day 150th of salt imposition and washed with distilled water to remove adherent soil. Dry weights (DWs) of phyllodes and roots were measured following the method described by Mostofa et al. (2015) (link).
4
Comparing Reproductive and Vegetative Ramets
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To investigate the difference in morphological characteristics and biomass allocation between reproductive and vegetative ramets of H. glabra, one ramet pair, consisting of one reproductive ramet and one vegetative ramet connected by a tillering node, was randomly collected at the edge of each clone at the seed-maturation stage in early June 2019. The length, width, and area of all leaves of each ramet were measured with a portable leaf area meter (AM350, ADC BioScientific Ltd., Herts, UK). The height of each ramet was measured. Each vegetative ramet was sorted into the leaves and sheath, while each reproductive ramet was separated into the leaves, sheath, stem, and panicle. The dry weight of all parts of each ramet was measured after oven-drying at 65 °C for 48 h.
5
Whitefly Infestation Effects on Plant Growth
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The measurement method of growth indexes is slightly modified with reference to Li et al. (2013) (link).
After being infested with whitefly for 0, 10, 20 and 30 d, plant height, internode length was measured, and number of leaves was recorded. There were 12 replicates for each experiment.
After feeding of whitefly for 30 d, third-leaf (lower leaf), fifth-leaf (middle leaf), and seventh-leaf (upper leaf) were taken for measurements. Leaf area was measured using portable laser leaf area meter (AM350, ADC BioScientific, Hoddesdon, UK). Using a punch (1 cm in diameter) leaf discs were taken from the seventh-leaf (upper leaf) and then dried in oven at 80 °C for 24 h. Dry weight was recorded and dry weight cm -2 was calculated. There were 12 replicates for each experiment.
After being infested with whitefly for 0, 10, 20 and 30 d, plant height, internode length was measured, and number of leaves was recorded. There were 12 replicates for each experiment.
After feeding of whitefly for 30 d, third-leaf (lower leaf), fifth-leaf (middle leaf), and seventh-leaf (upper leaf) were taken for measurements. Leaf area was measured using portable laser leaf area meter (AM350, ADC BioScientific, Hoddesdon, UK). Using a punch (1 cm in diameter) leaf discs were taken from the seventh-leaf (upper leaf) and then dried in oven at 80 °C for 24 h. Dry weight was recorded and dry weight cm -2 was calculated. There were 12 replicates for each experiment.
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