Sample turbidity (absorbance at 400 nm) was measured at 37 °C using the Tecan Spark multimode microplate reader with Te-CoolTM active temperature regulation. This was done in 10 mM Hepes buffer (pH 7.4) containing 100 mM NaCl, 1 mM DTT, 2 mM EDTA, and freshly prepared PEG 10,000 (PEG-10) (Sigma-Aldrich).
Peg 10
Manufactured by Merck Group
Sourced in United States
PEG-10 is a polyethylene glycol-based compound used in various laboratory applications. It functions as a solubilizing agent, emulsifier, and dispersant. The product specifications and performance characteristics are available upon request.
Automatically generated - may contain errors
2 protocols using peg 10
1
Turbidity Measurement of PEG-10 Solution
2
Synthesis of PEG-based Polymers
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Polyethylene glycol samples with molecular weights of 2 kDa, 6 kDa, and 10 kDa were supplied by Sigma-Aldrich Co. (Saint Louis, MO, USA); (referred as PEG2, PEG6, and PEG10, respectively). Hexamethylene diisocyanate (HMDI, 98%), triethylamine (TEA, 99%), toluene (99.5%), and tetrahydrofuran (THF, ≥99%) were also purchased from Sigma-Aldrich. Tecoflex™ SG-80A was obtained from Lubrizol (Wickliffe, OH, USA).
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