Emission filter wheels

Viral infections were imaged with Amersham Typhoon 5 scanner (Cytiva) with default Cy2 (excitation 488 nm, emission 520 nm) setting. Images of cells infected with SINV BFP-to-GFP edited virus were captured with Nikon Ti-Eclipse inverted microscope (Nikon Instruments) equipped with a SpectraX LED excitation module (Lumencor) and emission filter wheels (Prior Scientific). Fluorescence imaging used excitation/emission filters and dichroic mirrors for GFP and DAPI (Chroma Technology Corp.). Images were acquired with Plan Fluor 20Ph objective and an iXon 896 EM-CCD camera (Andor Technology Ltd.) in NIS-Elements software. Nucleic acid gels were stained with SYBR Gold (ThermoFisher Scientific) and imaged using Cy2 setting with Amersham Typhoon 5 scanner (Cytiva). Protein gels were stained with Coomassie stain (in-house), washed and imaged with IR-short default setting with Amersham Typhoon 5 scanner (Cytiva).

Viral infections were imaged with an Amersham Typhoon 5 scanner (Cytiva) with the default Cy2 (excitation, 488 nm; emission, 520 nm) setting. Images of cells infected with SINV BFP-to-GFP edited virus were captured with Nikon Ti-Eclipse inverted microscope (Nikon Instruments) equipped with a SpectraX light-emitting diode (LED) excitation module (Lumencor) and emission filter wheels (Prior Scientific). Fluorescence imaging used excitation/emission filters and dichroic mirrors for GFP and 4′,6-diamidino-2-phenylindole (DAPI) (Chroma Technology Corp.). Images were acquired with Plan Fluor 20Ph objective and an iXon 896 electron-multiplying charge-coupled device (EM-CCD) camera (Andor Technology Ltd.) in NIS-Elements software. Nucleic acid gels were stained with SYBR Gold (Thermo Fisher Scientific) and imaged using the Cy2 setting with an Amersham Typhoon 5 scanner (Cytiva). Protein gels were stained with Coomassie stain (in-house), washed, and imaged with infrared-short default setting with an Amersham Typhoon 5 scanner (Cytiva).