Mitmab ab120466

Biological particulate matter (PM), which is derived from pHrodo™ Red Staphylococcus aureus Bioparticles™ Conjugate for phagocytosis (A10010), was purchased from Invitrogen (Woltham, MA, USA). Oligomycin (ab141829), the PH domain inhibitor MiTMAB (ab120466) and OctMAB (ab120467) were purchased from Abcam (Cambridge, UK). DPI (81050) was purchased from Cayman Chemical Co. (Ann Arbor, Michigan, USA). PerCP Cy5.5-conjugated anti-human CD11b (65–0112-U100) was purchased from Tombo Biosciences (San Diego, CA, USA). Anti-mouse Ly6G particles (558111) were purchased from BD Biosciences (San Jose, CA, USA). Adenosine triphosphate ATP (017-21511) was purchased from Wako (Osaka, Japan). The IKK inhibitor VII (401486) was purchased from Calbiochem (Darmstadt, Germany). BAY 11-7085 (BML-EI279) was purchased from Enzo Life Sciences (Farmingdale, NY, USA). LY294002 (#9901S) was purchased from Cell Signaling Technology (Danvers, MA, USA). These inhibitors were dissolved in dimethyl sulfoxide (DMSO) and diluted in PBS before treatment. Bortezomib (7282) was purchased from Tocris (Minneapolis, MN, USA). MG-132 (474790) was purchased from Calbiochem. Bortezomib and MG-132 were dissolved in ethanol and diluted in PBS before treatment.

Human holo‐Tf (208‐18971) and hinokitiol (085‐06251) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Recombinant human EPO (873999) was purchased from Chugai Pharmaceutical Co., Ltd. (Tokyo, Japan). Percoll (17‐0891‐02) was obtained from GE Healthcare (Buckinghamshire, UK). FITC‐anti‐mouse CD44 (103006), PE‐conjugated anti‐mouse Ter119 (116208), PE/Cy7‐conjugated anti‐mouse CD71 (113812), Pacific blue‐conjugated anti‐mouse CD45 (103126), biotin‐conjugated anti‐mouse CD45 antibodies (103104), 7‐aminoactinomycin D (7‐AAD; 420404), MojoSort™ Streptavidin Nanobeads (480016), and MojoSort™ Magnet (480019) were purchased from BioLegend (San Diego, CA, USA). The anti‐TfR1 monoclonal antibody R17 208.2 (sc‐65883) was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Control rat IgM (14‐4341‐85), SYTO16 (S7578), and Cell‐Tak Cell and Tissue Adhesive (354240) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). RITC‐conjugated anti‐rabbit IgG antibody (SA00007‐2) was obtained from Proteintech (Rosemont, IL, USA). MitMAB (ab120466) and anti‐TfR1 antibody (ab84036) were obtained from Abcam (Cambridge, UK).