DU145 and LNCaP cells were obtained from American Type Culture Collection and PC3 were obtained from Dr. Leonard Deftos at UCSD, and maintained in RPMI 1640 media supplemented with 10% fetal bovine serum and 1× pen/strep and in 5% CO2 at 37°C. For spheroid assays, cells were maintained in RPMI 1640 media supplemented with 10% Gibco KnockOut Serum Replacement (KnockOut SR) from ThermoFisher Scientific.
Induced Pluripotent Stem (iPS) 87 cells (iPS87) were generated as previously described [28 (link), 29 (link)]. The iPS87 cells were grown on Mitomycin-C inactivated MEF feeder cells and maintained in KnockOut DMEM (Gibco) supplemented with 0.125% Bovine Serum Albumin (Sigma), 2% L-glutamine, 1% non-essential amino acids, 1% Fungizone/0.5% gentamycin 10% serum replacement (Gibco), 6.25 ng/mL bFGF (Peprotech), referred to as “ES+/+,” 5% CO2, 37°C [29 (link)].
Induced Pluripotent Stem (iPS) 87 cells (iPS87) were generated as previously described [28 (link), 29 (link)]. The iPS87 cells were grown on Mitomycin-C inactivated MEF feeder cells and maintained in KnockOut DMEM (Gibco) supplemented with 0.125% Bovine Serum Albumin (Sigma), 2% L-glutamine, 1% non-essential amino acids, 1% Fungizone/0.5% gentamycin 10% serum replacement (Gibco), 6.25 ng/mL bFGF (Peprotech), referred to as “ES+/+,” 5% CO2, 37°C [29 (link)].