Acacetin

All chemicals were obtained as pure commercial products and used without further purification. Acetylcholinesterase (AChE) from Electrophorus electricus, acetylthiocholine iodide, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), aluminum nitrate, butyrylcholinesterase (BChE) from equine serum, S-butyrylthiocholine chloride, 3-O-caffeoylquinic acid (chlorogenic acid), catechin, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH^•), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), ellagic acid, Folin-Ciocalteu phenol reagent, ferric chloride, galantamine hydrobromide, gallic acid, 4-hydroxybenzyl alcohol, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), myricetin, 2,4,6-tris(2-pyridyl)-1,3,5-triazine (TPTZ), and quercetin were purchased from Sigma Aldrich (Milan, Italy). LC-MS grade acetonitrile and formic acid were purchased from Merck (Darmstadt, Germany). Standards of myricetin-3-O-glucoside, quercetin-3-O-glucoside, and acacetin were purchased from Extrasynthese (Genay, France). HPLC grade water (18 MΩ·cm) was prepared by using a Millipore (Bedford, MA, USA) Milli-Q purification system.
Spectrophotometric determinations were obtained with an Ultrospec 2100 spectrophotometer (Biochrom Ltd., Cambridge, England) using cells with a 1 cm path length.

The standard compounds and reagents (all of analytical grade): MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), DMSO (dimethyl sulfoxide), gallic acid, Na₂CO₃, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), Folin-Ciocalteu’s reagent, resazurin, tetracycline, chloramphenicol, kanamycin, acetonitrile (LC-MS grade, gradient grade), formic acid, 4-methoxybenzoic acid, 4-hydroxy-3-methoxycinnamaldehyde, caffeic acid, quercetin, luteolin and pinostrobin PBS were purchased from Merck (Darmstadt, Germany). The standards of 4-hydroxybenzoic acid, p-coumaric acid, ferulic, isoferulic acid, pinobanksin, chrysin, sakuranetin, naringenin, apigenin, kaempferol, isorhamnetin, acacetin and pinocembrin were obtained from Extrasynthese (Genay, France) and galangin from Alfa Aesar, (Haverhill, MA, USA). The antibiotics: ampicillin, oxacillin, teicoplanin, gentamicin, amikacin, fusidic acid, erythromycin, mupirocin, rifampicin, levofloxacin, norfloxacin, linezolid, bacitracin were purchased from Argenda (Poland). Methanol, ethanol, vanillin, benzoic acid, cinnamic acid were purchased from (POCH, Gliwice, Poland). Ultrapure H₂O (18.0 MΩ) was obtained with a Milli-Q Advantage A10 system (Millipore, Billerica, MA, USA). The absorbance of the reaction mixture in Folin-Ciocalteu, DPPH assays were measured using a Genesys 20 spectrophotometer (Thermo Scientific, Waltham, MA, USA).

All chemicals were obtained as pure commercial products and used without further purification.
Acetonitrile, 3-O-caffeoylquinic acid (chlorogenic acid), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), fluorescein sodium salt (FL), 4-hydroxypyrazolo(3,4-d)pyrimidine (allopurinol), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), phosphoric acid 85% w/v, quercetin, xanthine, and xanthine oxidase from bovine milk were purchased from Sigma-Aldrich (Milan, Italy). Standards of kaempferol-3-O-glucoside, quercetin-3-O-glucoside, ellagic acid, and acacetin were purchased from Extrasynthese (Genay Cedex, France). HPLC grade water (MΩ•cm) was prepared by using a Millipore (Bedford, MA, USA) Milli-Q purification system. Acetonitrile, water, and formic acids (all of LC-MS grade) were purchased from Merck (Darmstadt, Germany).