Primary bone marrow aspirates or peripheral blood samples were collected from AML patients after informed consent or from excess samples obtained from de-identified AML subjects at diagnosis on a protocol approved by the Research Subjects Review Board of the University of Rochester. Mononuclear cells were isolated using Ficoll-Paque Plus (GE Healthcare Bio-Science AB) following the manufacturer's procedure and stored frozen in liquid N2. Primary AML cells were cultured in IMDM supplied with 10% HI-FBS and recombinant human (rh) stem-cell factor (SCF, 10 ng/mL), rh interleukin-3 (IL-3, 10 ng/mL), and rh FLT3-Ligand (10 ng/mL) (all from PeproTech). All cells were incubated in a humidified atmosphere at 37 °C in 5% CO2.
Recombinant human stem cell factor scf
Manufactured by Thermo Fisher Scientific
Recombinant human (rh) stem-cell factor (SCF) is a protein that plays a crucial role in the regulation and maintenance of stem cells. It functions as a growth factor, promoting the survival, proliferation, and differentiation of various types of stem cells, including hematopoietic stem cells and progenitor cells.
Automatically generated - may contain errors
Lab products found in correlation
Hi fbs, by Thermo Fisher Scientific (1 mentions)
Ficoll paque plus, by GE Healthcare (1 mentions)
Rhflt3 ligand, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Dasatinib, by Chemietek (1 mentions)
Nilotinib, by Chemietek (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Canertinib, by Chemietek (1 mentions)
Osi 906, by Chemietek (1 mentions)
Bibw2992, by Chemietek (1 mentions)
Bms 599626, by Chemietek (1 mentions)
2 protocols using recombinant human stem cell factor scf
1
Isolation and Culture of Primary AML Cells
2
Mab Specification and Reagents Used
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A specification of mAb used in this study is shown in Table 1 . The ErbB-targeting drugs canertinib, BIBW2992, and BMS-599626, the KIT kinase blockers nilotinib, midostaurin (PKC412) and dasatinib, and the insulin-like growth factor-1 receptor (IGF-1-R) blocker OSI-906, were from Chemietek (Indianapolis, IN). The irreversible ErbB inhibitor pelitinib and the CD33-targeting antibody-conjugate gemtuzumab ozogamicin (mylotarg) were kindly provided by Wyeth (Cambridge, MA). Recombinant human (rh) stem cell factor (SCF) was purchased from Peprotech (Rocky Hill, NJ), rh erythropoietin (EPO) and rh transforming growth factor-β1 (TGF-β1) from R&D Systems (Minneapolis, MN), and rh Heregulin-α (EGF-domain) from Sigma-Aldrich (St. Louis, MO). Dulbecco's modified eagle medium (DMEM) and fetal calf serum (FCS) were from Gibco Life Technologies (Gaithersburg, MD).
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