The apoptosis assays were run and analyzed with the help of Annexin V-FITC/PI Double Staining apoptosis detection kit (BB-4101, Bestbio, China). HaCaT cells were obtained using trypsinization 48 hours after intervention. Cells were then resuspended in binding buffer (1 × 106 cells/mL). Complying with the instructions of the manufacturer, cells were double-stained with FITC-Annexin V as well as propidium iodide (PI). Fluorescence intensity was measured using CytoFlex Analysis Flow Cytometer (Beckman Coulter) to detect early and late apoptosis of cells.
Annexin 5 fitc pi double staining apoptosis detection kit
Manufactured by BestBio
Sourced in China
The Annexin V-FITC/PI double staining apoptosis detection kit is a laboratory tool used to detect and quantify apoptosis in cell samples. It contains Annexin V-FITC, which binds to phosphatidylserine on the cell surface, and propidium iodide (PI), which stains the DNA of cells with compromised membranes. This kit allows for the identification of early apoptotic, late apoptotic, and necrotic cells through flow cytometric analysis.
Automatically generated - may contain errors
Lab products found in correlation
Cytoflex analysis flow cytometer, by Beckman Coulter (1 mentions)
Culture medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Anti bcl 2, by Proteintech (1 mentions)
Anti tfam, by Proteintech (1 mentions)
Anti sirt3, by Abcam (1 mentions)
Anti β actin, by Proteintech (1 mentions)
Tunel kit, by Beyotime (1 mentions)
Anti bax, by Proteintech (1 mentions)
Anti pgc 1α, by Proteintech (1 mentions)
Accuri c6, by BD (1 mentions)
5 protocols using annexin 5 fitc pi double staining apoptosis detection kit
1
Annexin V-FITC/PI Apoptosis Assay
2
Licorice-Derived Liquiritigenin Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Standard liquiritigenin of purity >98% was purchased from Aladdin Holdings Group Co., Ltd. (Shanghai, China). CP of over 98.5% purity (by HPLC) and the TUNEL kit were purchased from Beyotime Biotechnology (Shanghai, China). Annexin V-FITC/PI double staining apoptosis detection kit were obtained from BestBio (Shanghai, China). The antibodies used for Western blot analysis were as follows: anti-PGC-1α (Mouse, 1:1000, Catalog No.66369-1-Ig), anti-TFAM (Rabbit, 1:1000, Catalog No.22586-1-AP), anti-BCL-2 (Rabbit, 1:1000, Catalog No.26593-1-AP), anti-BAX (Mouse, 1:1000, Catalog No.60267-1-Ig), anti-β-actin (Mouse, 1:6000, Catalog No.66009-1-Ig), purchased from Protein Tech Group (Chicago, IL, USA), and anti-SIRT3 (Rabbit, 1:1000, Catalog No.ab189860), purchased from Abcam (Abcam, Cambridge, UK). Reagents related to cell culture such as culture medium, fetal bovine serum, and streptomycin and penicillin were purchased from Gibco (Shanghai, China).
3
Apoptosis Detection in MC3T3-E1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used the Annexin V-FITC/PI double staining apoptosis detection kit (BB-4101-50T, BestBio, Shanghai, China) and operated it according to the operating steps of the reagent instruction manual. Flow cytometry FC 500 MPL was used to determine each group's apoptosis rate of MC3T3-E1 cells.
4
Apoptosis Induction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MC38 cells were cultured in 6 well-plates at a cell density of 2 × 105 cells per well for 24 h. Then, the complete medium was replaced with a blank medium, and cells were irradiated with the abovementioned X-ray dose to obtain DTC. After 24 h, DTC was collected and stained with Annexin V-FITC/PI double staining apoptosis detection kit (BestBio, Nanjing, China) according to the manufacturer's instructions for flow cytometry analyzation (BD Accuri C6, New Jersey, USA). For FDT, cells were resuspended in Annexin V buffer solution at a cell density of 1 × 106 cells/mL and stained with Annexin V-FITC/PI double staining apoptosis detection kit for flow cytometry analyzation.
5
Apoptosis Detection in FLSs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Annexin V-FITC/PI double staining apoptosis detection kit was used to detect apoptosis (Bestbio, Shanghai). FLSs precipitation was collected by centrifugation, and was washed twice with PBS, and then resuspended with 400 µL of 1×Annexin V binding solution, and the cell density was adjusted to 1×10 6 cells/mL. 10 µL FITC labeled Annexin-V were added to FLSs suspension and incubated in the dark for 20 min. Then 5 µL of PI staining solution was added and incubated at 4 o C for 5 min in the dark. The apoptosis rate was detected by flow cytometry.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!