Nucline

Manufactured by Mediso

Sourced in Hungary

The Nucline is a laboratory equipment product designed for nuclear medicine applications. It serves as a platform for various nuclear imaging techniques. The core function of the Nucline is to enable the acquisition and processing of nuclear imaging data, facilitating diagnostic and research procedures in the field of nuclear medicine.

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Lab products found in correlation

Multicell imaging chamber, by Mediso (2 mentions) Interview fusion software, by Mediso (2 mentions) Nanoscan pet mri 1t, by Mediso (1 mentions) Vivoquant software, by Invicro (1 mentions) Nanoscan spect ct, by Mediso (1 mentions) Interview fusion, by Mediso (1 mentions) Nanoscan pet ct scanner, by Mediso (1 mentions)

Close spelling variants of this product

Nucline 2.03 Software Nucline v2.01 Nucline TH Nucline acquisition software Nucline nanoScan software Nucline software

6 protocols using nucline

Tumor targeting with Ga-68 labeled NGR peptides

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Healthy control (n=3/radiopharmaceutical), He/De (n=5/radiopharmaceutical) and Ne/De (n=5/radiopharmaceutical) tumor-bearing F-344 rats were anaesthetized with 3% Forane using a dedicated small animal anaesthesia device and were injected with 12.3±1.2 MBq of 68 Ga-NOTA-c(NGR) or 68 Ga-NODAGA-c(NGR) or 68 Ga-NODAGA-c(NGR) (MG1) or 68 Ga-NODAGA-c(NGR) (MG2) in 150 μL saline via the lateral tail vein 11±1 after tumor cell inoculation. 90 min after radiotracer injection whole-body PET/MRI scans were peformed using the preclinical nanoScan PET/MRI 1T device (Mediso LTD., Hungary). Special rat imaging chamber (MultiCell Imaging Chamber, Mediso LTD., Hungary) was used to eliminate the movement of rats and to maintain a permanent body temperature. For the anatomical localization of tissues and organs T1 weighted MRI scans (Material map) were performed. For the image reconstruction (3D-OSEM) and analysis of the PET images Nucline and InterView™ FUSION software (Mediso LTD., Hungary) were used, respectively.
Standardized uptake value (SUV) was calculated using the following formula: SUV= [ROI activity (MBq/mL)]/[injected activity (MBq)/animal weight (g)].

Kis A., Dénes N., Szabó J.P., Arató V., Jószai I., Enyedi K.N., Lakatos S., Garai I., Mező G., Kertész I, & Trencsényi G. (2020). In vivo assessment of aminopeptidase N (APN/CD13) specificity of different ⁶⁸Ga-labelled NGR derivatives using PET/MRI imaging. International journal of pharmaceutics, 589.

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Multimodal Imaging of Radiotracers in Mice

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Selected animals from the biodistribution cohort (n = 1-2/ group) were subjected to in vivo imaging using a PET/MR 3 T or SPECT/CT scanner (both nanoscan series, Mediso) prior to reaching their endpoint. PET imaging of 68 Ga-Trivehexin injected mice was performed for 15 min (75-90 min p.i.), preceded by an MR scan for anatomical correlation. SPECT imaging of 177 Lu-D0301 injected mice was performed for 60 min (23-24 h p.i.) and preceded by a CT scan for anatomical correlation. Reconstruction, image analysis and quantification of PET/MR and SPECT/CT data and image analysis were performed using Nucline and Interview fusion software (both Mediso). MRI sequence parameters: GRE (gradient echo) 3D 0.25 mm iso, NEX: 3 (number of averages), T R : 20 ms, T E : 4.0 ms. PET reconstruction parameters: TT3D, It:4, Ss:6, 400-600 keV, 1:3, R:0.0005, M:24.

Stangl S., Nguyen N.T., Brosch-Lenz J., Šimeček J., Weber W.A., Kossatz S, & Notni J. (2024). Efficiency of succinylated gelatin and amino acid infusions for kidney uptake reduction of radiolabeled αvβ6-integrin targeting peptides: considerations on clinical safety profiles. European journal of nuclear medicine and molecular imaging.

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Multimodal Imaging of COLO205 Tumor Response

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COLO205 tumor-bearing mice (N = 6) underwent CT+PET scanning, which was performed consecutively in the same imaging session (total scan time 2.5 h), before and 24 h following treatment with MEDI3039. Helical CT data were acquired for anatomical reference and for attenuation correction using Nucline (V2.01, Mediso). Images were acquired with a semicircular scan, with 180 projections. The X-ray energy was set to 55 kVp with 600 milliseconds exposure and 1:4 image binning. Images were reconstructed using Butterworth filtering with an isotropic voxel size of 213 μm. PET images, with a nominal isotropic resolution of 0.6 mm, were reconstructed using a dynamic protocol (Tera-Tomo 3D (Mediso) algorithm), energy window 400–600 keV, coincidence modes 1–5, full detector model, normal regularization, 2 iterations, 6 subsets, with attenuation and random scatter correction. Images were analyzed in VivoQuant^® software (vs. 4.0 patch 1, InviCRO, Needham, MA, USA). A dynamic PET acquisition lasting 120 min was initiated 30 s prior to intravenous injection of 3.7 ± 1.2 MBq 68Ga-C2Am (1075 ± 382 μg protein/kg body weight; 10 mL/kg; SA = 0.15 ± 0.05 MBq/μg protein). Scans were reconstructed into 23 time bins (4 between 0–1 min; 4 between 1–5 min; 11 between 5–60 min and 4 between 60–120 min).

Bulat F., Hesse F., Attili B., Solanki C., Mendichovszky I.A., Aigbirhio F., Leeper F.J., Brindle K.M, & Neves A.A. (2023). Preclinical PET Imaging of Tumor Cell Death following Therapy Using Gallium-68-Labeled C2Am. Cancers, 15(5), 1564.

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In Vivo Whole-Body CT Imaging

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For the in vivo imaging, mice were anesthetized by 3% isoflurane (Forane) with a dedicated small animal anesthesia device. Whole-body CT scans were acquired with the preclinical nanoScan SPECT/CT (Mediso Ltd, Hungary) scanner using the following acquisition parameters: X-ray tube voltage 60 kVp, current 86 mA; exposure time 170 ms per projection; voxel size: 1 × 1 mm. For the CT image reconstruction and analysis, the Nucline and InterView FUSION software (Mediso Ltd., Hungary) was used, respectively.

Gönczi M., Ráduly Z., Szabó L., Fodor J., Telek A., Dobrosi N., Balogh N., Szentesi P., Kis G., Antal M., Trencsenyi G., Dienes B, & Csernoch L. (2022). Septin7 is indispensable for proper skeletal muscle architecture and function. eLife, 11, e75863.

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In Vivo Gold Nanoparticle Biodistribution

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Mice were anesthetized with an isoflurane/O₂ mix and tail vein cannulated for the delivery of approximately 0.1 ml of gold nanoparticles per 25 g of mouse weight. Animal temperature was maintained at 37°C, with monitoring and maintenance of respiration at 60 breaths/min. Mice were placed in the supine position 4 h after injection of gold nanoparticles and whole body CT scans were acquired using a nanoScanPET/CT scanner (Mediso) with a 50 kVP X-ray source and 300 ms exposure time in 720 projections with an acquisition time of 15 min. CT images were reconstructed in voxel size 68 x 68 x 68 μm using Nucline (Mediso) software. Image analysis and 3D visualization was performed using VivoQuant (inviCRO version 1.23patch3) software.

Castillo S.D., Tzouanacou E., Zaw-Thin M., Berenjeno I.M., Parker V.E., Chivite I., Milà-Guasch M., Pearce W., Solomon I., Angulo-Urarte A., Figueiredo A.M., Dewhurst R.E., Knox R.G., Clark G.R., Scudamore C.L., Badar A., Kalber T.L., Foster J., Stuckey D.J., David A.L., Phillips W.A., Lythgoe M.F., Wilson V., Semple R.K., Sebire N.J., Kinsler V.A., Graupera M, & Vanhaesebroeck B. (2016). Somatic Activating Mutations in Pik3ca Cause Sporadic Venous Malformations in Mice and Humans. Science translational medicine, 8(332), 332ra43.

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Multimodal Imaging of Tumor Angiogenesis and Hypoxia

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He/De tumor-bearing animals were anaesthetized with dedicated small animal anaesthesia device (Tec3 Isoflurane Vaporizer) and were injected with 10.2 ± 1.1 MBq of ⁶⁸Ga-NODAGA-[c(RGD)]₂ or ⁶⁸Ga-NOTA-c(NGR) (angiogenesis imaging) or ⁶⁸Ga-DOTA-nitroimidazole (hypoxia imaging) in 150 μl saline via the lateral tail vein. 90 min after radiotracer injection, whole-body PET/MRI scans were peformed with a preclinical nanoScan PET/MRI device (Mediso LTD., Hungary). A special rat imaging chamber (MultiCell Imaging Chamber, Mediso LTD., Hungary) was used to eliminate the movement of rats and to maintain a permanent body temperature. For the anatomical localization of tissues and organs, T1-weighted MRI scans (GRE EXT multi-FOV; phase: 144; TR/TE 15/2 ms; FOV 60 mm; excitation number: 2) were performed. For the image reconstruction (3D-OSEM) and analysis of the PET images, Nucline and InterView™ FUSION software (Mediso LTD., Hungary) were used, respectively. Standardized uptake value (SUV) was calculated through the following formula: SUV = [ROI activity (MBq/ml)]/[injected activity (MBq)/animal weight (g)].

Kis A., Szabó J.P., Dénes N., Vágner A., Nagy G., Garai I., Fekete A., Szikra D., Hajdu I., Matolay O., Méhes G., Mező G., Kertész I, & Trencsényi G. (2020). In Vivo Imaging of Hypoxia and Neoangiogenesis in Experimental Syngeneic Hepatocellular Carcinoma Tumor Model Using Positron Emission Tomography. BioMed Research International, 2020, 4952372.

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