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Sorbitan monolaurate span 20

Manufactured by Merck Group
Sourced in United States

Sorbitan monolaurate (Span 20) is a non-ionic surfactant commonly used in various laboratory applications. It acts as an emulsifier, dispersant, and wetting agent. The product's core function is to facilitate the formation and stabilization of emulsions and dispersions in aqueous and non-aqueous systems.

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7 protocols using sorbitan monolaurate span 20

1

Viral Tissue Culture Reagents

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Sorbitan monolaurate (Span 20) was purchased from Sigma-Aldrich, USA. Polyoxyethylene sorbitan monooleate (Tween 80) was obtained from El-Nasr pharmaceutical chemical company, Egypt. Sodium pentane sulfonic acid, isopropyl myristate (IPM), and ethanol (99%) were purchased from Jamjoom Pharma, Saudi Arabia. All of the reagents of the viral tissue culture were obtained from King Abdulaziz University hospital, Jeddah, Saudi Arabia.
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2

Letrozole-Loaded Nanoparticle Formulations

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Letrozole was kindly donated by Tofigh Daru (Tehran, Iran). Sorbitan monolaurate (Span20, Cat# S6635), Sorbitan monostearate (Span60, Cat# S7710), Sorbitan monooleate (Span80, Cat# S6760), Cholesterol (CHOL, Cat# C8667), dicetylphosphate (DCP, Cat# 850042P), Sodium dodecyl sulfate (SDS, Cat# L3771) and dialysis membrane (MWCO 12kDa, Cat# D6191), Nile red and Coumarin 6 were obtained from Sigma Aldrich (USA). Dimethyl sulfoxide (DMSO, Cat# 109678), chloroform (Cat# 107024), Amicon (Ultra-15-Membrane, MWCO 30kDa, Cat# C82301) were purchased from Merck (Germany). Trypsin-EDTA (Cat# 15400054), Trypan blue (Cat# 15250061), RPMI-1640 culture medium, DMEM (Dulbecco’s Modified Eagle culture medium, Cat# 11965092), phosphate-buffered saline (PBS, Cat# 10010049), fetal bovine serum (FBS, Cat# 16000044), MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (Cat# M6494) and penicillin/streptomycin (PS, Cat# 10378016) 100 X were purchased from Gibco (USA). The MDA-MB-231, MCF-7, and MCF10A cell lines were obtained from Pasteur Cell Bank (Iran). The Annexin V-FITC flow cytometric kit was obtained from Affymetrix Biosciences (USA). RNA extraction and cDNA synthesis kits were purchased from Transgene Biotech, China (Cat#. ER101-01 and AE301-02).
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3

Synthesis of Silver Nanoparticles and Turmeric Nanoemulsions

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Silver nitrate (AgNO3) and surfactants polysorbate (Tween 20) and sorbitan monolaurate (Span 20) required for preparing AgNPs and TNE, respectively, were procured from Sigma-Aldrich (Sigma-Aldrich Chemical Co, St. Louis, MO, USA). The curcumin-removed turmeric oleoresin (CRTO) was obtained from Sami Labs Limited (Bangalore, India). Two hybrid varieties of watermelon seeds, Riverside (diploid) and Maxima (triploid) were obtained from Origene America Inc. (Alamo, TX, USA).
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4

Immobilized Urease Biosensor Development

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Sodium alginate (mol. wt. 120,000–190,000), urease, and sorbitan monolaurate (Span 20) were purchased from Sigma-Aldrich, New Delhi, India. Calcium chloride, manganous chloride, ferrous chloride, cobalt chloride, sodium chloride, Nessler’s reagent, and urea were supplied by Fisher Scientific, New Delhi, India. Hexane was supplied by CDH, New Delhi, India. All chemicals were of analytical grade (AR) and were used as received. Triple distilled water was used throughout the experiments.
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5

Formulation and Evaluation of Pharmaceutical Tablet

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Sodium lauryl sulfate (SLS), Sodium hydroxide, and sodium dihydrogen phosphate dihydrate analytical grades were purchased from Merck, Germany. Ethanol (EtOH), methanol (MeOH) and ethyl acetate (EtAc) were (HPLC grades) and were purchased from J.T.Baker, Netherlands. Methylene chloride and ammonia solution (25%) were analytical grades and were purchased from Elnasr Chemicals, Egypt.
Copovidone, talc powder, sodium stearyl fumarate, sorbitan monolaurate (span-20) and propylene glycol monolaurate were pharmaceutical grades produced by Sigma-Aldrich, Germany and were kindly supplied and certified from EIPICo, Egypt.
PAR, RIT and OMB (analytical grades) were kindly supplied and certified by EIPICo., Egypt (structures shown in Fig. 1). De-ionized water was produced in-house by Millipore water purification system.

Chemical structure of drugs under study (1. PAR, 2. RIT, 3. OMB).

Fig. 1
Qurevo® pharmaceutical tablet dosage form, containing (12.5 mg, 75 mg and 50 mg of OMB, PAR and RIT, respectively) was purchased from Egyptian market, (Batch number 1051458; produced by abbvie).
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6

Curcumin-Loaded Pluronic F-127 Nanoparticles

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Pluronic F-127® was a gift from BASF chemical company (St. Louis, MO, USA). 1-Ethyl-3-[3-dimethylaminopropyl]-carbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide (sulfo-NHS), tetrahydrofuran (THF), dimethylaminopyridine (DMAP) succinic anhydride polysorbate 80 (TWEEN 80), Sorbitan monolaurate (Span 20), glycerine, propylene glycol, polyethylene glycol 400 (PEG 400), and isopropyl myristate (IPM) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethanol, mEthanol, and carbon tetrachloride were obtained from Sigma-Aldrich (Steinheim, Germany). Curcumin was obtained from Merck (Darmstadt, Germany). KLVFF peptide was synthesized and purified by PEPMIC (Suzhou, China). Monobasic potassium phosphate was purchased from RCI Labscan (Bangkok, Thailand). Sodium hydroxide was obtained from KEMAUS (New South Wales, Australia).
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7

CBD-Loaded Self-Emulsifying Drug Delivery

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Sesame oil, glyceryl trioctanoate, polyoxyethylenesorbitan trioleate (Tween® 85, Sigma-Aldrich, Dorset, UK), polyethylene glycol sorbitan monooleate (Tween® 80), sorbitan monolaurate (Span® 20, Sigma-Aldrich, Dorset, UK), d-α-Tocopherol polyethylene glycol 1000 (TPGS), sodium hydroxide solution (NaOH, 1 M), l-α-phosphatidylcholine (~60%, from egg yolk), Trizma® (Sigma-Aldrich, Dorset, UK) maleate, sodium taurocholate hydrate, pancreatin from porcine pancreas (8 × USP specifications), sodium taurocholate hydrate, probucol, 4,4-dichlorodiphenyltrichloroethane (DDT) and serum triglyceride determination kit were all purchased from Sigma-Aldrich (Dorset, UK). Sodium chloride and anhydrous calcium chloride were purchased from Fisher Scientific (Leicester, UK). Cannabidiol (CBD, ≥98%) was purchased from THC Pharm (Frankfurt, Germany). Rat plasma was purchased from Sera Laboratories International (West Sussex, UK). All solvents were purchased from Fisher Scientific (Leicester, UK), and were of HPLC grade.
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