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Ultrascan 895 ccd camera

Manufactured by Ametek

The Ultrascan 895 CCD camera is a high-performance imaging device designed for laboratory and scientific applications. It features a charge-coupled device (CCD) sensor that captures visual data with high resolution and sensitivity. The camera is capable of capturing detailed images and digital data for various analytical and research purposes, but a more detailed description of its intended use is not available.

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4 protocols using ultrascan 895 ccd camera

1

Negative Staining of L-NP Complexes

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Negative staining of the L-NP complexes was performed as described previously (75 (link)). Purified samples at approximately 0.02 to 0.05 mg/ml were applied to glow-discharged carbon-coated copper grids (Ted Pella) and stained with 0.75% (wt/vol) uranyl formate. Samples were imaged using a Philips CM10 microscope operated at 100 kV with a tungsten filament and UltraScan 894 charge-coupled device (CCD) camera (Gatan), or a Tecnai T12 microscope operated at 120 kV with a lanthanum hexaboride filament and UltraScan 895 CCD camera (Gatan). Images were collected at a magnification of 54,000× with an approximate defocus of −1.5 μm.
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2

Negative Staining Electron Microscopy

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Carbon-coated copper grids (Electron Microscopy Sciences, #CF200-Cu) were glow discharged at 30 mA for 30 s. A 4 μl aliquot of IFT-A sample (0.02 mg/mL) was applied to the grid. After incubating for 1 min, the grid was washed twice with 10 mM HEPES pH 7.4 and twice with 1.5% Uranyl formate followed by staining with 1.5% Uranyl formate for 2 min. The grids were imaged using either a 100 kV CM10 (Philips) or a 120 kV Tecnai T12 (Thermo Fisher Scientific) microscope. Images were recorded using an Ultrascan 895 CCD camera (Gatan).
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3

Cryo-EM Imaging of Mastigonemes and C. reinhardtii

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Isolated mastigonemes (0.2–0.7 mg/mL) or whole C. reinhardtii cells (107 cells/mL) were applied to glow-discharged carbon-coated copper grids (Electron Microscopy Sciences). After 1 min incubation, the grids were blotted, immediately washed twice with 1.5% uranyl formate solution, and stained for 90 s with 1.5% uranyl formate solution. The grids were examined using a 120 kV Tecnai T12 (Thermo Fisher Scientific) microscope. Images were recorded using an Ultrascan 895 CCD camera (Gatan).
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4

Copper Grid Preparation for Electron Microscopy

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Carbon-coated copper grids (Electron Microscopy Sciences, #CF400-Cu) were glow discharged at 30 mA for 30 s. A 4 μL aliquot of a mMIB1 full length sample (0.02 mg/mL) was applied to the grid. After incubating for 1 min, the grid was washed twice with 1.5% Uranyl formate followed by staining with 1.5% Uranyl formate for 2 min. The grids were imaged using a 120 kV Tecnai T12 (Thermo Fisher Scientific) microscope. Images were recorded using an Ultrascan 895 CCD camera (Gatan).
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