Log In Sign up
The largest database of trusted experimental protocols

Mcs00

Manufactured by R&D Systems
Visit Supplier
Sourced in United States

The MCS00 is a laboratory equipment used for the measurement and analysis of various samples. It is designed to provide precise and reliable data to researchers and scientists.

Automatically generated - may contain errors

Lab products found in correlation

Luminex technology, by Merck Group (3 mentions) Interleukin 6 (il 6), by R&D Systems (3 mentions) Mcx120, by R&D Systems (2 mentions) Il 18, by Thermo Fisher Scientific (2 mentions) Tnf α, by BioLegend (2 mentions) Ary006, by R&D Systems (1 mentions) Ary015, by R&D Systems (1 mentions) Mlb00b, by R&D Systems (1 mentions) Ab229440, by R&D Systems (1 mentions) Cholesterol e assay, by Fujifilm (1 mentions) Csb e10371m, by Cusabio (1 mentions) Mcx130, by R&D Systems (1 mentions) Spectramax m5, by Molecular Devices (1 mentions) Mmv00, by R&D Systems (1 mentions) Mb100b, by R&D Systems (1 mentions) Mp200, by R&D Systems (1 mentions) Mck00, by R&D Systems (1 mentions) Mob00, by R&D Systems (1 mentions) Most00, by R&D Systems (1 mentions) Mip 1α, by R&D Systems (1 mentions)

13 protocols using mcs00

1

Profiling Secreted Proteins in Murine Models

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasmas and conditioned media were analyzed for mouse G-CSF (MCS00; R&D Systems), IL-1β (MLB00B; R&D Systems), MMP9 (MMP900B; R&D Systems) and MMP-8 (Uscn Life science Inc.) according to manufacturer's instructions. Conditioned media from splenocytes or tumor cell lines were analyzed for the secretion of various proteins using mouse angiogenesis array (ARY015; R&D Systems) and mouse cytokine array (ARY006; R&D Systems) according to manufacturer's instructions.
Spiegel A., Brooks M.W., Houshyar S., Reinhardt F., Ardolino M., Fessler E., Chen M.B., Krall J.A., DeCock J., Zervantonakis I.K., Iannello A., Iwamoto Y., Cortez-Retamozo V., Kamm R.D., Pittet M.J., Raulet D.H, & Weinberg R.A. (2016). Neutrophils suppress intraluminal NK-mediated tumor cell clearance and enhance extravasation of disseminated carcinoma cells. Cancer discovery, 6(6), 630-649.
+ Open protocol
+ Expand
2

Mouse Plasma Biomarker Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse plasma was isolated through centrifugation of blood at 12 000 g for 10 min at 4°C. IL-18, IL-1β, and G-CSF plasma levels were measured by ELISA (MBL International, 7625) and (Abcam, ab229440 and R&D systems MCS00), respectively. Total plasma cholesterol was determined using a cholesterol E assay (Wako, cat. no. 999–02601).
Yalcinkaya M., Fotakis P., Liu W., Endo-Umeda K., Dou H., Abramowicz S., Xiao T., Libby P., Wang N., Tall A.R, & Westerterp M. (2022). Cholesterol accumulation in macrophages drives NETosis in atherosclerotic plaques via IL-1β secretion. Cardiovascular Research, 119(4), 969-981.
+ Open protocol
+ Expand
3

Cytokine Profiling in Lung Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse IL-1β (88-7013-76, Invitrogen, Carlsbad, CA, USA), IL-23 (88-7230-22, eBioscience), s100a8 (MBS2504318, Mybiosourse), s1009a (MBS2886839, Mybiosourse, San Diego, CA, USA), procalcitonin (PCT) (CSB-E10371m, Cusabio, College Park, MD, USA), and G-CSF (MCS00, R&D Systems) ELISA kits were used to assess cytokine levels in lung homogenates. All analyses were performed according to the manufacturer's instructions.
Lee S., Kim G.L., Kim N.Y., Kim S.J., Ghosh P, & Rhee D.K. (2018). ATF3 Stimulates IL-17A by Regulating Intracellular Ca2+/ROS-Dependent IL-1β Activation During Streptococcus pneumoniae Infection. Frontiers in Immunology, 9, 1954.
+ Open protocol
+ Expand
4

Quantifying G-CSF, CXCL13, and Bv8/PROK2 in Mouse and Human Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse sera were diluted fivefold, and G-CSF and CXCL13 levels were measured using specific ELISA kits (MCS00 and MCX130 respectively, R&D Systems). For G-CSF ELISA in conditioned media of mouse CRC cell lines, 1.0 × 105 cells were seeded in six-well plates with 1 mL of Dulbecco’s Modified Eagle Medium containing 10% fetal bovine serum, and cultured for 24 h. The absorbance was measured at 450 nm using the plate reader SpectraMax M5 (Molecular Devices). For human Bv8/PROK2, assay plates were coated with anti-Bv8/PROK2 antibody (1 μg/mL, clone 3B8) at 4 °C overnight, and after a brief wash with wash buffer (0.05% Tween-20 in phosphate-buffered saline), human plasma samples were applied and incubated for 2 h. Anti-Bv8/PROK2 (clone 3F1) was used as detection antibody. Animal studies were approved and performed in compliance with guidelines by the University of California San Diego (UCSD) Institutional Animal Care and Use Committee.
Itatani Y., Yamamoto T., Zhong C., Molinolo A.A., Ruppel J., Hegde P., Taketo M.M, & Ferrara N. (2020). Suppressing neutrophil-dependent angiogenesis abrogates resistance to anti-VEGF antibody in a genetic model of colorectal cancer. Proceedings of the National Academy of Sciences of the United States of America, 117(35), 21598-21608.
+ Open protocol
+ Expand
5

Comprehensive Mouse Plasma Proteome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse plasma protein concentration was measured for PlGF2 (dilution factor: 1), VEGF (dilution factor: 2), SDF1α (dilution factor: 1), G-CSF (dilution factor: 1), HGF (dilution factor: 1), TGFβ1 (dilution factor: 60), OPN (dilution factor: 100), sVEGFR2 (dilution factor: 20), CSF (dilution factor: 2), and Leptin (dilution factor: 10). All ELISA kits were purchased from R&D Systems (catalogue numbers: MP200, MMV00, MCX120, MCS00, DY2207, MB100B, MOST00, MVR200, MCK00, MOB00, respectively) and used according to manufacturer’s instructions. Protein levels after treatment were normalized to corresponding vehicle controls, and fold changes in log2 scale were graphed.
Mastri M., Lee C.R., Tracz A., Kerbel R.S., Dolan M., Shi Y, & Ebos J.M. (2018). Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors. Molecular cancer therapeutics, 17(7), 1602-1612.
+ Open protocol
+ Expand
6

Cytokine Profiling in Spinal Cord and Colon

Check if the same lab product or an alternative is used in the 5 most similar protocols
Both spinal cord homogenates and colon-secreted cytokines were analyzed by Enzyme–Linked Immunosorbent Assay (ELISA, MCS00, R&D Systems, Minneapolis, MN) or by multiplex analyte assay using Luminex technology (EMD Millipore, Darmstadt, Germany) according to manufacturers’ protocols. IL-18 (Invitrogen BMS618–3), IL-6 (R&D DY406) and TNFα (Biolegend 430904) were measured using ELISA assays according to manufacturer’s instructions. For colon-secreted cytokines, excised colons were washed and flushed with PBS containing 2× penicillin/streptomycin. The distal-most 1 cm2 colon sections were cultured for 15 hr in RPMI media containing 2× penicillin/streptomycin at 37°C. Supernatants were collected, cleared of debris by centrifugation and assessed for cytokines by Luminex analyses.
Chou W.C., Guo Z., Guo H., Chen L., Zhang G., Liang K., Xie L., Tan X., Gibson S.A., Rampanelli E., Wang Y., Montgomery S.A., Brickey W.J., Deng M., Freeman L., Zhang S., Su M.A., Chen X., Wan Y.Y, & Ting J.P. (2021). AIM2 in regulatory T cells restrains autoimmune diseases. Nature, 591(7849), 300-305.
+ Open protocol
+ Expand
7

Cytokine and Chemokine Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sera of adult mice or cell supernatants were analyzed by Luminex Panel (BioRad). The following cytokines and chemokines were analyzed from cell supernatants: MIP1α (R&D Systems, MMA00), IL-6 (R&D Systems, D6050), G-CSF (R&D Systems, MCS00).
Kist M., Kőműves L.G., Goncharov T., Dugger D.L., Yu C., Roose-Girma M., Newton K., Webster J.D, & Vucic D. (2020). Impaired RIPK1 ubiquitination sensitizes mice to TNF toxicity and inflammatory cell death. Cell Death and Differentiation, 28(3), 985-1000.
+ Open protocol
+ Expand
8

Quantifying G-CSF and IL-13 Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
ELISA quantification of candidate secreted factors’ protein levels in the blood was performed with a granulocyte-colony stimulating factor (G-CSF) mouse ELISA Kit (MCS00, R&D Systems, Minneapolis, MN, USA) and a Mouse IL-13 ELISA Kit (M1300CB, R&D systems), according to the manufacturer’s instructions.
Achlaug L., Awwad L., Langier Goncalves I., Goldenberg T, & Aronheim A. (2023). Tumor Growth Ameliorates Cardiac Dysfunction and Suppresses Fibrosis in a Mouse Model for Duchenne Muscular Dystrophy. International Journal of Molecular Sciences, 24(16), 12595.
+ Open protocol
+ Expand
9

Measuring Cytokine Levels in Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood samples were collected. Plasma was separated by centrifugation, and monocyte chemoattractant protein-1 (MCP-1), macrophage-colony stimulating factor (M-CSF), and granulocyte-colony stimulating factor (G-CSF) were measured using ELISA kits (MJE00, MMC00, and MCS00, respectively; R&D systems, Minneapolis, MN, USA) according to the manufacturer's instructions.
La Rose A.M., Bazioti V., Hoogerland J.A., Svendsen A.F., Groenen A.G., van Faassen M., Rutten M.G., Kloosterhuis N.J., Dethmers-Ausema B., Nijland J.H., Mithieux G., Rajas F., Kuipers F., Lukens M.V., Soehnlein O., Oosterveer M.H, & Westerterp M. (2021). Hepatocyte-specific glucose-6-phosphatase deficiency disturbs platelet aggregation and decreases blood monocytes upon fasting-induced hypoglycemia. Molecular Metabolism, 53, 101265.
+ Open protocol
+ Expand
10

Cytokine Profiling in Spinal Cord and Colon

Check if the same lab product or an alternative is used in the 5 most similar protocols
Both spinal cord homogenates and colon-secreted cytokines were analyzed by Enzyme–Linked Immunosorbent Assay (ELISA, MCS00, R&D Systems, Minneapolis, MN) or by multiplex analyte assay using Luminex technology (EMD Millipore, Darmstadt, Germany) according to manufacturers’ protocols. IL-18 (Invitrogen BMS618–3), IL-6 (R&D DY406) and TNFα (Biolegend 430904) were measured using ELISA assays according to manufacturer’s instructions. For colon-secreted cytokines, excised colons were washed and flushed with PBS containing 2× penicillin/streptomycin. The distal-most 1 cm2 colon sections were cultured for 15 hr in RPMI media containing 2× penicillin/streptomycin at 37°C. Supernatants were collected, cleared of debris by centrifugation and assessed for cytokines by Luminex analyses.
Chou W.C., Guo Z., Guo H., Chen L., Zhang G., Liang K., Xie L., Tan X., Gibson S.A., Rampanelli E., Wang Y., Montgomery S.A., Brickey W.J., Deng M., Freeman L., Zhang S., Su M.A., Chen X., Wan Y.Y, & Ting J.P. (2021). AIM2 in regulatory T cells restrains autoimmune diseases. Nature, 591(7849), 300-305.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!