Bafilomycin a1

Manufactured by CSNpharm

Sourced in United States

Bafilomycin A1 is a macrolide antibiotic compound. It functions as a specific and potent inhibitor of vacuolar-type H+-ATPases (V-ATPases).

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Lab products found in correlation

Sbi 0206965, by CSNpharm (2 mentions) Doramapimod, by CSNpharm (2 mentions) Mg132, by Merck Group (2 mentions) Bortezomib, by Selleck Chemicals (1 mentions) Ferrostatin 1, by CSNpharm (1 mentions) Z vad fmk, by CSNpharm (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Caki 1, by American Type Culture Collection (1 mentions)

3 protocols using bafilomycin a1

Evaluation of Autophagy Inhibitors

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MG132 (Merck Millipore, 474790); Bafilomycin A1 (CSNpharm, CSN10374); Bortezomib (Selleck, S1013); CX-4945 (MCE, HY-HY-50855); BX795 (MCE, HY-10514); SBI-0206965 (CSNpharm, CSN16884); Doramapimod (CSNpharm, CSN10856); Wortmannin (MCE, HY-10197).

Zhang C., Duan Y., Huang C, & Li L. (2023). Inhibition of SQSTM1 S403 phosphorylation facilitates the aggresome formation of ubiquitinated proteins during proteasome dysfunction. Cellular & Molecular Biology Letters, 28, 85.

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RCC Cell Lines and Pharmacological Inhibitors

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RCC cell lines 786-O, Caki-1, A498, 769-P and ACHN and the normal cell line HK-2 were obtained from the American Type Culture Collection (Manassas, VA, USA). All cell lines were cultured in the recommended medium supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco, Australia) at 37 °C with 5% CO₂. Chemical reagents, including ferrostatin-1 (CSN12654), z-VAD-FMK (CSN19230), necrostatin 1 (CSN11637), bafilomycin A1 (CSN10374), VX-765 (CSN15837), UNC0638 (CSN16350) and chaetocin (CSN19229) were provided by CSNpharm (Chicago, IL, USA).

Wang J., Yin X., He W., Xue W., Zhang J, & Huang Y. (2020). SUV39H1 deficiency suppresses clear cell renal cell carcinoma growth by inducing ferroptosis. Acta Pharmaceutica Sinica. B, 11(2), 406-419.

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Autophagy and Kinase Inhibitor Screening

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The following reagents were used: MG132 (Merck Millipore, 474790); Bafilomycin A1 (CSNpharm, CSN10374); SBI-0206965 (CSNpharm, CSN16884); Bortezomib (CSNpharm, CSN10115); Doramapimod (CSNpharm, CSN10856); Wortmannin (MCE, HY-10197); SB203580 (MCE, HY-10256); SB202190 (HY-10295); Rapamycin (MCE, HY-10219); Torin 2 (MCE, HY-13002); Asciminib (Topscience, T5177); BRAF inhibitor (MCE, HY-10247); Chloroquine (MCE, HY-17589A).

Zhang C., Huang C., Xia H., Xu H., Tang Q, & Bi F. (2022). Autophagic sequestration of SQSTM1 disrupts the aggresome formation of ubiquitinated proteins during proteasome inhibition. Cell Death & Disease, 13(7), 615.

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