The flow cytometry assay was performed to measure the levels of apoptosis by using ApoScreen Annexin V kit (Southern Biotech, Birmingham, AL, USA) according to the manufacturer’s protocol. The number of stained cells was assessed by a flow cytometer (FACS Aria II, BD Biosciences).
Aposcreen annexin 5 kit
Manufactured by Southern Biotech
Sourced in United States
The ApoScreen Annexin V kit is a laboratory product designed to detect and quantify apoptosis, a type of programmed cell death. It utilizes Annexin V, a protein that binds to phosphatidylserine, a molecule exposed on the surface of apoptotic cells. The kit provides the necessary reagents and protocols to perform this analysis.
Automatically generated - may contain errors
Lab products found in correlation
9 protocols using aposcreen annexin 5 kit
1
Apoptosis Measurement by Flow Cytometry
2
Annexin V-based Apoptosis and Necrosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A flow cytometry assay was performed to investigate the cell apoptosis and necrosis rates using an ApoScreen Annexin V kit (Southern Biotech, Birmingham, AL, USA), according to the manufacturer's protocol. Briefly, HT-29 cells were digested using 0.1% trypsin and then resuspended in cold binding buffer (10 mM HEPES, pH 7.4, 140 mM NaCl, 2.5 mM CaCl2 and 0.1% BSA) at concentrations between 105 and 106 cells/ml. Following this, labeled Annexin V (10 µl) was added to 100 µl of the cell suspension. Propidium iodide (PI) solution (10 µl) and binding buffer (380 µl) were then added to the cell suspension following incubation for 15 min on ice. Subsequently, the number of stained cells was investigated using a BD FACSAriaII flow cytometer (CXP software, version 2.2; FC500 flow cytometer; Beckman Coulter, Inc., Brea, CA, USA) (32 (link)).
3
Quantifying Rat Cell Death in Co-culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell death was induced by growing rCM/cFb either mono-cultured or co-cultured with MSC or human dermal fibroblast in serum-free media for 20 h before incubation with Paclitaxel/Taxol (Cedarlane, Burlington, ON) at a final concentration of 2.5 μM, 24 h for cells cultured on plate, and 10 μM, 30 h for cells cultured in collagen patches. Next, single cell suspensions were prepared and stained with anti-human CD44 antibody (APC, eBioscience) for negative selection of rat cells (CD44-ve) before staining with annexin-V using an ApoScreen Annexin-V kit (SouthernBiotech) following manufacturer’s instructions. Cell death was quantified in CD44(-ve) rat cells by flow cytometry.
4
Apoptosis and Necrosis Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Flow cytometry was performed to determine the levels of early-stage apoptosis, late-stage apoptosis and necrosis using the ApoScreen Annexin V kit (SouthernBiotech, Birmingham, AL, USA) according to the manufacturer’s protocol. Briefly, PC12 cells exposed to various concentrations of glutamate were digested with 0.25% trypsin, washed with cold PBS, and resuspended in cold 1 × binding buffer at concentrations between 1.0 × 106 and 1.0 × 107 cells/mL. A 5-μL aliquot of labeled Annexin V was added to 100 μL of the cell suspension. The number of stained cells was assessed immediately on a flow cytometer (FACS Aria II, BD Biosciences, Franklin Lakes, NJ, USA).
5
Flow Cytometry Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell apoptosis was measured by flow cytometry assay. Cells were treated with media containing 10% FBS ± inhibitors for 24 h. The ApoScreen Annexin V kit (Southern Biotech, Birmingham, AL, USA) was used for flow cytometry (FACS Aria II, BD Biosciences) to detect the level of apoptosis according to the protocol of the manufacturer and the data were analyzed by FlowJo software.
6
Apoptosis Measurement by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The flow cytometry assay was performed to measure the levels of apoptosis by using ApoScreen Annexin V kit (Southern Biotech, Birmingham, AL, USA) according to the manufacturer's protocol. Briefly, medium was collected followed by washing with cold PBS twice and the cold PBS was collected as well. Then cells were digested with 0.25% trypsin-EDTA to resuspend cells to a concentration between 1 × 106 and 1 × 107 cells/mL in cold 1X binding buffer. Later 5 µL fluorescence-labeled Annexin V was added into 100 µL suspended cells. After incubation on ice for 15 min in the dark, 200 µL binding buffer and 5 µL 7-AAD solution were added into the cell suspensions. The number of stained cells was assessed immediately by a flow cytometer (FACS Aria II, BD Biosciences).
7
Quantification of Necrosis and Apoptosis in PC12 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Flow cytometry assay was conducted to determine the levels of necrosis and apoptosis of PC12 cells, and the ApoScreen Annexin V kit (SouthernBiotech, Birmingham, AL, USA) was used. Briefly, differentiated PC12 cells were harvested by 0.25% trypsin solution. After washes with PBS, the cells were incubated with 100 μL cold 1X binding buffer and 5 μL Annexin V, which were put in ice for 15 min. Finally, 200 μL 1X binding buffer and 5 μL 7-AAD were mixed into the samples. The levels of necrosis and apoptosis of PC12 cells were measured by a flow cytometer (FACSAria II, BD Biosciences).
8
Apoptosis and Necrosis Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The degrees of both apoptosis and necrosis were measured using ApoScreen Annexin V kit (Southern-Biotech, Birmingham, AL, USA). Briefly, cells were resuspended in pre-chilled binding buffer at concentrations of 5 × 106 cells/mL. Annexin V solution was added into cell suspension at a dilution of 1:10. After incubation on ice for 15 min, 7-AAD solution was added into cell suspension at a dilution of 1:40. Further analysis was performed on a flow cytometer (BD FACSAriaII).
9
Apoptosis and Necrosis Assessment in Daudi and OCI-LY8 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Flow cytometry was performed to investigate the degree of apoptosis and necrosis of Daudi and OCI-LY8 cell lines. Briefly, samples from each experimental group were collected, washed with ice-cold PBS, stained with propidium iodide (PI) and ApoScreen Annexin V kit (Southern Biotechnology, Birmingham, AL, USA) according to the manufacturer’s instructions prior to FACScan flow cytometry (BD FACSAriaII, Becton, Dickinson and Co., Franklin Lakes, NJ, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!