Mdm2 m4308

Manufactured by Merck Group

Sourced in United States

MDM2 (M4308) is a laboratory reagent produced by Merck Group. It serves as a protein that plays a role in the regulation of the tumor suppressor protein p53. The core function of this product is to facilitate research and experimentation related to the MDM2-p53 interaction, which is of interest in various biological and medical fields.

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Lab products found in correlation

P p53 ser15, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) P rb ser795, by Cell Signaling Technology (1 mentions) P p38 mapk thr180 tyr182, by Cell Signaling Technology (1 mentions) 8 oxoguanine, by Abcam (1 mentions) Flag tag (flag), by Merck Group (1 mentions) P21 sc 6246, by Santa Cruz Biotechnology (1 mentions) P53 sc 126, by Santa Cruz Biotechnology (1 mentions) C myc, by Merck Group (1 mentions) Wave1, by Abcam (1 mentions) N wasp, by Cell Signaling Technology (1 mentions)

3 protocols using mdm2 m4308

Western Blot Protocol for Protein Analysis

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Western blotting was carried out in compliance with the standard protocol. Antibodies used were: p-P53(ser15) (#9286), p-Rb(ser795) (#9301), p-p38MAPK(Thr180/tyr182) (#9212), extracellular signal-regulated kinases 1/2 (#4695), E-cadherin (#3195), β-catenin (#8481), tubulin (#2128) (Cell Signaling Technology, Danvers, MA, USA), GAPDH (ap7873a) and MMP2 (am1844a) (ABGENT, San Diego, CA, USA), DHRS2 (PA5-25258) (Thermo Fisher Scientific), MDM2 (M4308) (Sigma-Aldrich) and 8-oxoguanine (AB20646) (Abcam, Cambridge, UK). pCDH was bought from System Biosciences (Palo Alto, CA, USA). pCDH-DHRS2-V1 and DHRS2-V2 were constructed and sequenced. pLKO.1-DHRS2-shRNA targeting both variants was bought from Sigma-Aldrich.

Zhou Y., Wang L., Ban X., Zeng T., Zhu Y., Li M., Guan X.Y, & Li Y. (2017). DHRS2 inhibits cell growth and motility in esophageal squamous cell carcinoma. Oncogene, 37(8), 1086-1094.

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Western Blot Analysis of Viral Proteins

Cited 1 time

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Detailed protocol for Western blot analysis of protein samples was described previously [41 (link)]. The anti-ORF50 polyclonal antibody used in the study was generated in our laboratory using purified ORF50(333–691) as an immunogen. Antibodies to MDM2 (M4308; Sigma-Aldrich, St. Louis, MO, USA), K8 (sc-57889; Santa Cruz, Dallas, TX, USA), ORF45 (sc-53883; Santa Cruz, Dallas, TX, USA), K8.1 (sc-65446; Santa Cruz, Dallas, TX, USA), FLAG (A8592; Sigma-Aldrich, St. Louis, MO, USA), p53 (sc-126; Santa Cruz, Dallas, TX, USA), Sp1 (CS200631; Millipore, Burlington, MA, USA), p21 (sc-6246; Santa Cruz, Dallas, TX, USA), and actin (sc-47778; Santa Cruz, Dallas, TX, USA) were purchased commercially.

Lin C.I., Wang S.S., Hung C.H., Chang P.J, & Chen L.W. (2022). Kaposi’s Sarcoma-Associated Herpesvirus ORF50 Protein Represses Cellular MDM2 Expression via Suppressing the Sp1- and p53-Mediated Transactivation. International Journal of Molecular Sciences, 23(15), 8673.

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Protein Detection Protocol: Immunofluorescence and Western Blotting

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The following primary antibodies were used in this study for immunofluorescence microscopy and western blotting: c-myc (Sigma-Aldrich), cortactin 4F11 (Millipore), Glyceraldehyde-3-Phosphate (GAPDH) (MAB374) (Millipore), p53 (Cell signaling), Rac1 (Cell Biolabs), PTEN (Cell Signaling), Cdc42 (Cell Biolabs), WAVE1 (Abcam), WAVE2 (Abcam), N-WASP (Cell signaling), and MDM2 (M4308) (Sigma-Aldrich), GTP-Rac (Neweast Biosciences). Alexa Fluor 488- and Alex Fluor 568-conjugated secondary antibodies (Molecular Probes). Tetramethyl rhodamines isothiocynate (TRITC)-conjugated phalloidin (P1951) (Sigma-Aldrich) was added along with the secondary antibodies to stain for F-Actin. Western blotting was performed using the primary antibodies cited above as well as specific anti-mouse and anti-rabbit horseradish-peroxidase conjugated secondary antibodies (Millipore).

Payne L.J., Eves R.L., Jia L, & Mak A.S. (2014). p53 Down Regulates PDGF-Induced Formation of Circular Dorsal Ruffles in Rat Aortic Smooth Muscle Cells. PLoS ONE, 9(9), e108257.

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