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Sp8 white light inverted confocal microscope

Manufactured by Leica

The SP8 White Light inverted confocal microscope is a high-performance lab equipment designed for advanced microscopy applications. It features a white light laser excitation source that provides a wide range of excitation wavelengths, allowing for flexible and versatile imaging. The system is configured as an inverted microscope, enabling the examination of samples from below.

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2 protocols using sp8 white light inverted confocal microscope

1

Multimodal Imaging of Organoids

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Brightfield imaging of organoids was performed using a Leica M80 stereo microscope (Leica Microsystems) and a Leica DMIL LED microscope (Leica Microsystems). H&E images were taken using a Leica DM400B LED microscope (Leica Microsystems). Karyotypes and IF staining were imaged using a SP8 White Light inverted confocal microscope (Leica Microsystems) or with a Leica DMI3000 fluorescent inverted microscope (Leica Microsystems). Optical sections were acquired at 3 μm intervals. Images were acquired with Leica application suite X Software and processed using Fiji.
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2

Confocal Microscopy Imaging Protocol

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Samples were imaged on an SP8 White Light inverted confocal microscope (Leica Microsystems) through a 10× or 20× objective using Leica application suite X Software. Optical sections were acquired at 2 µm intervals. Images were processed using Fiji.
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