Mice were given free access to food and drinking water, and blood was collected from the abdominal vena cava between 11:00 and 15:00 under isoflurane anaesthesia after ghrelin administration/stress exposure. Animals were exsanguinated under anaesthesia by transecting the abdominal aorta and the inferior vena cava after blood collection. Collected blood was centrifuged immediately to collect the plasma. To determine blood ghrelin levels, 10% HCl (1 N) was added to the plasma. Plasma hormone concentrations were measured using the Active/Des‐acyl Ghrelin ELISA Kit (LSI Medience Corporation, Tokyo, Japan), Rat/Mouse Growth Hormone ELISA Kit (Merck Millipore, Darmstadt, Germany), and 17β‐estradiol high‐sensitivity ELISA kit (Enzo Life Sciences, PA, USA). For the measurement of growth hormone, mice were fasted before the measurement and blood was collected between 10:30 and 11:30 when the pulse had stabilized (Steyn et al., 2011 ). These measurements were performed according to the protocol attached to the product.
17β estradiol high sensitivity elisa kit
Manufactured by Enzo Life Sciences
Sourced in United States
The 17β-estradiol high sensitivity ELISA kit is a quantitative in vitro diagnostic assay designed to measure the concentration of 17β-estradiol, a steroid hormone, in biological samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) principle to detect and quantify the levels of 17β-estradiol present in the samples.
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Lab products found in correlation
Rat mouse growth hormone elisa kit, by Merck Group (2 mentions)
Camp complete elisa kit, by Enzo Life Sciences (1 mentions)
Dc protein assay kit, by Bio-Rad (1 mentions)
Lh elisa kit, by Enzo Life Sciences (1 mentions)
Testosterone elisa kit, by Enzo Life Sciences (1 mentions)
Mouse rat igf 1 quantikine elisa kit, by R&D Systems (1 mentions)
5 protocols using 17β estradiol high sensitivity elisa kit
1
Blood Collection and Hormone Measurements in Mice
2
Quantifying Cell Signaling Molecules
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The concentration of E2, bone morphogenetic protein (BMP)-2, or BMP-6 in the medium was determined using a 17β-Estradiol high-sensitivity ELISA kit (Enzo Life Sciences, Farmingdale, NY, USA), Quantikine ELISA BMP-2 Immunoassay (R&D systems, Minneapolis, MN, USA) or Rat BMP-6 ELISA Kit (Novus Biologicals, Centennial, CO, USA). The concentration of cyclic adenosine monophosphate (cAMP) or total protein in the cell lysate was determined using a cAMP complete ELISA kit (Enzo Life Sciences) or a DC Protein Assay kit (Bio-Rad, Hercules CA, USA).
3
Quantification of Plasma Estradiol Levels
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The plasma samples first underwent a liquid-liquid extraction using diethyl ether to eliminate interfering compounds in the plasma as previously described [75 (link)]. Following diethyl ether extraction, samples were reconstituted using sample buffer contained in the 17β-estradiol high sensitivity ELISA kit (Enzo Life Sciences, Cat. No. AD 901 174), which was used to determine concentration of circulating E2 levels. Absorbance was measured on a BioTek (Winooski, VT) Synergy HT plate reader. The circulating plasma E2 concentrations in the E2 and vehicle animals were 56.68 (+/− 20.29) and 36.10 (+/− 9.70) pg/mL respectively (Figure 1b ). These E2 levels were consistent with physiological levels observed during late diestrous/early proestrous and with levels achieved in postmenopausal women following HT [76 (link)]. The intra- and inter-assay %CV was 4.23 and 6.85 pg/ml respectively.
4
Serum Hormone Measurement in Rats
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Throughout the entire experimental period, weekly blood samples were obtained from tail vein of all rats. Serum was obtained and kept at −20°C until hormonal measurements. The concentrations of estradiol (E2) (17β-Estradiol high sensitivity ELISA kit; Catalog #: ADI-900–174) and luteinizing hormone (LH) (LH ELISA KIT; Catalog #: ENZ-KIT107) were measured using ELISA kits (Enzo Life Science) following the protocols provided by the manufacturer.
5
Quantification of Endocrine Biomarkers
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The plasma concentrations of IGF-1, testosterone, 17β-estradiol, GH and luteinizing hormone (LH) were quantified using Mouse/Rat IGF-1 Quantikine ELISA kit (MG100, R&D Systems, Wiesbaden, Germany), Testosterone ELISA kit (ADI-900-065, Enzo Life Sciences, Farmingdale, NY, USA), 17β-estradiol high sensitivity ELISA kit (ADI-900-174, Enzo Life Sciences), Rat/Mouse Growth Hormone ELISA kit (EZRMGH-45k, Millipore, Darmstadt, Germany) and Rat Luteinizing Hormone ELISA kit (MBS764675, MyBioSource, San Diego, CA, USA), respectively. Analyses with each kit were performed according to the manufacturer’s instruction.
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