The PI3K inhibitor copanlisib, TGX-221, alpelisib, idelalisib, and LY294002 were purchased from MedChemExpress (Princeton, New Jersey, USA). The PI3K inhibitor pictilisib, the ALK inhibitor crizotinib and NVP-TAE684 were obtained from ADOOQ (Irvine, California, USA). The antibodies against ALK, phospho-ERK1/2, Akt1/2, N-Myc, GAPDH, and beta-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, California, USA). Antibodies targeting Phospho-Akt (Ser473), Phospho-S6 Ribosomal Protein (Ser235/236), S6 Ribosomal Protein, and anti-Phospho-Histone H3 (Ser10) were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). Other antibodies used in this study included anti-MAPK1/2 (Milipore, NG1946), anti-β-Tubulin antibody (Abcam, Ab6046).
Tgx 221
Manufactured by MedChemExpress
Sourced in United States
TGX-221 is a selective inhibitor of the p110β isoform of phosphoinositide 3-kinase (PI3K). It is a small molecule compound used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Anti β tubulin antibody, by Abcam (1 mentions)
Anti phospho histone h3 ser10, by Cell Signaling Technology (1 mentions)
Alpelisib, by MedChemExpress (1 mentions)
Copanlisib, by MedChemExpress (1 mentions)
Idelalisib, by MedChemExpress (1 mentions)
N myc, by Santa Cruz Biotechnology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Ly294002, by MedChemExpress (1 mentions)
Latrunculin a, by Bio-Techne (1 mentions)
Y 27632 dihydrochloride, by Bio-Techne (1 mentions)
Bio 5192, by R&D Systems (1 mentions)
2 protocols using tgx 221
1
Profiling PI3K and ALK Inhibitors
2
Inhibition of Integrin and Cytoskeletal Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were treated with BIO 5192 (20 nM, 45 min; R&D Systems) to inhibit the VLA-4 integrin; latrunculin A (1 µM, 1 h; Tocris Bioscience) to inhibit actin polymerization; Blebb (50 µM, 10 min; MedChemExpress) to inhibit nonmuscle myosin II; Y-27632 dihydrochloride (10 µM, 1 h; Tocris Bioscience) to inhibit ROCK signaling; LY (5 µM, 1 h; MedChemExpress), BAY (Copanlisib; 64 nM, 1 h), CAL-101 (Idelalisib; 50 nM, 1 h), MLN1117 (Serabelisib; 150 nM, 1 h), and TGX-221 (85 nM, 1 h) to inhibit PI3K; and MK (5 µM, 1 h; MedChemExpress) and GSK2141795 (1 µM, 1 h) to inhibit Akt activity. Vehicle solvents were used accordingly as controls.
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