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Quantikine elisa human il 17 immunoassay kit

Manufactured by R&D Systems
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Sourced in United States

The Quantikine ELISA Human IL-17 Immunoassay Kit is a quantitative sandwich enzyme immunoassay designed to measure human interleukin 17 (IL-17) levels in cell culture supernates, serum, and plasma.

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2 protocols using quantikine elisa human il 17 immunoassay kit

1

Quantification of IL-17A Cytokine Secretion

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Quantikine ELISA Human IL-17 Immunoassay Kit (R&D, Minnesota, USA) was utilized according to the manufacturer’s protocol to measure the amount of IL-17A cytokine secreted by differentiating cells after 5 hours and 2 days, 4 days and 6 days of the induction period. All samples were measured in duplicate at 450 nm.
Bahmani L., Baghi M., Peymani M., Javeri A, & Ghaedi K. (2023). The PBX1/miR-141-miR-200a/EGR2/SOCS3 Axis; Integrative Analysis of Interaction Networks to Discover the Possible Mechanism of MiR-141 and MiR-200a-Mediated Th17 Cell Differentiation. Iranian Journal of Biotechnology, 21(1), e3211.
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2

IL-17 Cytokine Production in Th17 Cells

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Culture supernatants were collected 5 hours, 2 days, 4 days, and 6 days after suspending naïve CD4+ T cells in human Th17 differentiation media to check IL‐17 cytokine content. Enzyme‐linked immunosorbent assay (ELISA) was performed in duplicate using Quantikine ELISA Human IL‐17 Immunoassay kit (R&D) according to the manufacturer's protocols. Optical density values were measured by ELISA microplate reader (Awareness) at a wavelength of 450 nm.
Shirani F., Baghi M., Rostamian Delavar M., Shoaraye Nejati A., Eshaghiyan A., Nasr‐Esfahani M.H., Peymani M, & Ghaedi K. (2020). Upregulation of miR‐9 and miR‐193b over human Th17 cell differentiation. Molecular Genetics & Genomic Medicine, 8(12), e1538.
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