Glucose dextrose

All reagents for microfluidic
assays and isolation and culture of islets were obtained from Sigma-Aldrich
(St. Louis, MO, U.S.A.) unless otherwise stated. NaOH, KCl, Tween-20,
KCl, and HF were from EMD Chemicals (San Diego, CA, U.S.A.). Glucose
(dextrose), RPMI 1640, and gentamicin sulfate were from Thermo Fisher
Scientific (Waltham, MA, U.S.A.). Collagenase P (from Clostrdium histolyticum) was acquired from Roche
Diagnostics (Indianapolis, IN, U.S.A.). Cosmic Calf Serum was acquired
from GE Healthcare Bio-Sciences (Pittsburgh, PA, U.S.A.). Monoclonal
Ab_Ins was purchased from Meridian Life Science, Inc. (Saco,
ME, U.S.A.). All solutions were made with Milli-Q (Millipore, Bedford,
MA, U.S.A.) 18 MΩ·cm ultrapure water and filtered using
0.2 μm nylon syringe filters (Pall Corporation, Port Washington,
NY, U.S.A.). Immunoassay reagents (Ins* and Ab_Ins) were
prepared in TEAT-40 composed of 25 mM Tricine, 40 mM NaCl, 1 mM EDTA
at pH 7.4 with an additional 0.1% Tween-20 (w/v) and 1 mg mL^–1 BSA. A balanced salt solution (BSS) made to pH 7.4 was used for
preparing Ins standards and for islet perfusion. BSS was composed
of 125 mM NaCl, 2.4 mM CaCl₂, 1.2 mM MgCl₂,
5.9 mM KCl, 25 mM HEPES, 1 mg mL^–1 BSA, and 3, 11,
or 12 mM glucose. Reagents for isolation and culture of murine islets
were prepared as previously described.^{36 (link)}