Zeta sizer software v6

Liposomes were characterized by dynamic light scattering (DLS) to obtain information about the size and the polydispersity of liposomes. First, 1 mL of the liposome’s solution at the concentration obtained by the synthesis process was directly inserted into a disposable 1 cm beam plastic cell using a Zetasizer Nano ZS equipment (Malvern Instruments, Malvern, UK) and the Malvern Zetasizer software v6.34. Each sample was measured 3 times at 25 °C. SEM was used to confirm the morphology and the aggregation state of liposomes. To perform SEM, 20 μL of the sample at the concentration obtained in the synthesis process was put on a circular coverslip at room temperature and let dry overnight protected from direct sunlight. The coverslips containing the dried samples were fixed in a metallic sample holder with two-sided tape. The sample holder was inserted into the anode stage of an Emitech K550 (London, UK) sputter coater. The coater was vacuum closed, and the samples were gold coated for 1.5 min. Samples were observed using a Hitachi S-2700 (Tokyo, Japan) scanning electron microscope, with an accelerating voltage of 20 kV using various magnifications until reached a good image of liposomes.