Ki 67 mab 0672

MDA‐MB‐231 tumor bearing mice (50–100 mm³) were randomly divided into four groups (n = 5), intravenously injected with PBS and NPs‐Bev ([Gd³⁺] = 40 × 10⁻⁶ m kg⁻¹), and irradiated (or not) with X‐rays on days 0 and 6. Irradiated mice received X‐ray RT (6 Gy × 2) at 12 h after injection, and tumor volumes were measured at consecutive 3 days intervals. At day 21, mice were humanely sacrificed and tumor volumes measured. Tumor tissues were excised, embedded in paraffin, sectioned into 4 µm slices for H&E, IHC stained with Ki‐67 (MAB‐0672, MXB Biotechnologies, China) and Caspase 3 (200270‐T08, SinoBiological, China), and images analyzed using ImageJ software.

Paclitaxel (NSC 125 973), cisplatin (s1166), and doxorubicin (s1208) were purchased from Selleck. Actinomycin D (HY‐17559) and rapamycin (HY‐10219) were purchased from MCE. CQ (C6628) was purchased from Sigma. Antibodies used in immunoblotting: mTOR (2972S, 1:1000), p‐mTOR (Ser2448) (5536S, 1:1000), SQSTM1/p62 (88588S, 1:1000), BECN1 (D40C5, 1:1000), LC3 (3868S, 1:1000) were purchased from Cell Signaling Technologies. Anti‐REDD1 (10638‐1‐AP, 1:1000), anti‐AUF1 (12770‐1‐AP, 1:1000), anti‐ZFP36 (12737‐1‐AP, 1:1000), anti‐KHSRP (55409‐1‐AP, 1:1000), anti‐β‐actin (20536‐1‐AP,1:5000), were purchased from Proteintech. GAPDH (GB11002) was purchased from servicebio. LC3 (L7543, 1:2000) was purchased from Sigma. Ki67 (MAB‐0672) was purchased from MXB Biotechnologies. Anti‐H3K27 (ab4729, abcam), anti‐TCF4 (22337‐1‐AP, proteintech) and normal IgG (2729, CST) were used in the CHIP assay. Normal IgG/Peroxidase‐conjugated AffiniPure Goat Anti‐Rabbit/Mouse IgG (H+L) was purchased from Jackson Immuno Research.