Dextran from leuconostoc spp 500 000

Dextran from Leuconostoc spp. 500 000 (Sigma-Aldrich), Ficoll-Paque Plus d = 1.077 g/ml (GE Healthcare,), human eosinophil isolation kit (MiltenyiBiotec), DPBS (Dulbecco’s phosphate-buffered saline, Gibco), 10% fetal bovine serum (FBS, Gibco), penicillin–streptomycin (10 000 U/mL, Thermo-Fisher Scientific), Roswell Park Memorial Institute (RPMI) 1640 medium with L-glutamine and 25 mmol/l HEPES (Gibco), EDTA (Sigma-Aldrich), butyric acid (BA, Sigma-Aldrich); TNF-α (Tumour Necrosis Factor, Sigma-Aldrich), Il-1β (Interleukin 1β, Sigma-Aldrich) and LPSs (lipopolysaccharides, Sigma-Aldrich).

EPO solution (LeeBiosolutions, Maryland Heights, MO, USA), MPO solution (LeeBiosolutions, Maryland Heights, MO, USA), EPO powder (Sigma-Aldrich, St. Louis, MO, USA), MPO powder (LeeBiosolution, Maryland Heights, MO, USA), dextran from Leuconostoc spp. 500,000 (Sigma-Aldrich, St. Louis, MO, USA), Ficoll-Paque Plus d = 1.077 g/mL (GE Healthcare, Chicago, IL, USA), human eosinophil isolation kit (MiltenyiBiotec, Bergisch Gladbach, Germany), DPBS (Dulbecco’s phosphate-buffered saline, Gibco, ThermoFisher, Waltham, MA, USA), 10% fetal bovine serum (FBS, Gibco, ThermoFisher, Waltham, MA, USA), penicillin-streptomycin (10,000 U/mL, ThermoFisher Scientific, Waltham, MA, USA), Roswell Park Memorial Institute (RPMI) 1640 medium with L-glutamine and 25 mmol/l HEPES (Gibco), EDTA (Sigma-Aldrich, St. Louis, MO, USA).
Reference eosinophil peroxidase (EPO, Munich, Germany) and myeloperoxidase (MPO) were purchased as lyophilized powders and solutions (LeeBiosolutions, Maryland Heights, MO, USA). EPO solution (≥98% purity) was dissolved in 0.1 M sodium phosphate solution with 0.4 M sodium chloride (pH 7.5). MPO solution (≥96% purity) was dissolved in 50 mM sodium acetate and 100 mM sodium chloride solution with preservative (bromo-nitro-dioxane, pH 6.0).