Hifairtm 2 1st strand cdna synthesis kit

Manufactured by Yeasen

Sourced in China

The HifairTM II 1st Strand cDNA Synthesis Kit is a laboratory equipment product designed for the synthesis of first-strand cDNA from RNA samples. It facilitates the reverse transcription process, a critical step in various molecular biology applications.

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Lab products found in correlation

Nanodrop 2000, by Thermo Fisher Scientific (4 mentions) Abi7500 real time pcr amplifier, by Thermo Fisher Scientific (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Sybr green master mix, by Yeasen (2 mentions)

3 protocols using hifairtm 2 1st strand cdna synthesis kit

RNA Extraction and miRNA Quantification

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Total RNA was isolated from cells and EVs using Trizol reagent (Invitrogen, Life Technologies, Carlsbad, CA, USA). Concentration and quality of RNA was measured by Nanodrop2000 (Thermo Fisher). miRNAs were reverse transcribed using the HifairTM II 1st Strand cDNA Synthesis Kit (Yeasen). Quantitative real-time PCR analysis was performed in ABI7500 real-time PCR amplifier (Applied Biosystems, Waltham, MA, USA) using SYBR Green Master Mix (Yeasen). U6 or cel-miR-39 was used as control, and results were analyzed using the 2^−ΔΔct calculation method.

Li C., Qin F., Wang W., Ni Y., Gao M., Guo M, & Sun G. (2021). hnRNPA2B1-Mediated Extracellular Vesicles Sorting of miR-122-5p Potentially Promotes Lung Cancer Progression. International Journal of Molecular Sciences, 22(23), 12866.

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Total RNA Extraction and qRT-PCR Analysis

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Total RNA was isolated from cells and EVs using Trizol reagent (Invitrogen, Life Technologies, USA). The concentration and quality were assessed using the Nanodrop2000 (Thermo Fisher Scientific). RNA was reverse transcribed using the HifairTM II 1st Strand cDNA Synthesis Kit (Yeasen, China). Quantitative real-time PCR analysis was performed on the ABI7500 real-time PCR amplifier (Applied Biosystems, USA) using SYBR Green Master Mix (Yeasen, China). U6 or cel-miR-39 was used as a control, and results were analyzed using the 2^–ΔΔct method.

Li C., Wang W., Sun Y., Ni Y., Qin F., Li X., Wang T., Guo M, & Sun G. (2022). Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression. Journal of Experimental & Clinical Cancer Research : CR, 41, 136.

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m1A site detection by RT-PCR

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1 µg total RNA was reverse transcribed by HifairTM II 1st Strand cDNA Synthesis Kit (Yeasen). The region flanking m¹A site was amplified by plasmid specific primer pair #23 and #24. The mismatch site was measured by sanger sequencing using primer #23.

Fang L., Wang W., Li G., Zhang L., Li J., Gan D., Yang J., Tang Y., Ding Z., Zhang M., Zhang W., Deng D., Song Z., Zhu Q., Cui H., Hu Y, & Chen W. (2020). CIGAR‐seq, a CRISPR/Cas‐based method for unbiased screening of novel mRNA modification regulators. Molecular Systems Biology, 16(11), e10025.

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