Mouse igg2b

Manufactured by Southern Biotech

Mouse IgG2b is an immunoglobulin G (IgG) isotype produced by mouse B cells. It is a laboratory reagent used in various immunological assays and research applications.

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Lab products found in correlation

Mouse igg2a, by Southern Biotech (2 mentions) Gp130, by Santa Cruz Biotechnology (1 mentions) Filgotinib, by Selleck Chemicals (1 mentions) Hrp conjugated secondary antibody, by Southern Biotech (1 mentions) Mouse igg1, by Southern Biotech (1 mentions) Ps9 gsk3β, by Cell Signaling Technology (1 mentions) Py705 stat3, by Cell Signaling Technology (1 mentions) Alexa conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Stattic, by Selleck Chemicals (1 mentions) Ps727 stat3, by Cell Signaling Technology (1 mentions) Jam c, by R&D Systems (1 mentions) Ruxolitinib, by Selleck Chemicals (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Human igg, by Jackson ImmunoResearch (1 mentions) Mouse igg1 isotype control antibody clone mopc 21, by BioXCell (1 mentions)

2 protocols using mouse igg2b

Antibody Detection and Signaling Pathways

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The following primary antibodies were used (dilution 1:1,000 for Western blot, 1:200 for immunofluorescence and flow cytometry): GAPDH (mouse SC-25778; Santa Cruz Biotechnology), GP130 (rabbit SC-656 for Western blot, mouse SC-376280 for immunofluorescence; Santa Cruz Biotechnology; and mouse ab34324 for flow cytometry; Abcam), APLNR (mouse FAB8561R and MAB856; R&D Systems), JAMC (mouse FAB11891A; R&D Systems), STAT3 (rabbit 9132; Cell Signaling), pS727-STAT3 (rabbit 9134; Cell Signaling), pY705-STAT3 (rabbit 9145; Cell Signaling), pS235/S236-S6 (rabbit 2211 for Western blot and rabbit 5316 for flow cytometry; Cell Signaling), pS9-GSK3β (rabbit 9336; Cell Signaling), ELMOD1 (rabbit ab127541; Abcam), RAB5 (rabbit ab218624; Abcam) and RAB7 (rabbit 9367; Cell Signaling). HRP-conjugated secondary antibodies (anti-rabbit, mouse Ig, mouse IgG1, mouse IgG2a, and mouse IgG2b) were purchased from Southern Biotech. Alexa-conjugated secondary antibodies were from Life Technologies. FLAER (Alexa Fluor 488–conjugated proaerolysin) was purchased from Cedarlane, MBCD was from Sigma-Aldrich, and Stattic, ruxolitinib, and filgotinib were all from Selleckchem. [Pyr1]-Apelin-13 was resuspended and aliquoted according to the supplier’s recommendation (Phoenix).

Trillet K., Jacobs K.A., André-Grégoire G., Thys A., Maghe C., Cruard J., Minvielle S., Diest S.G., Montagnac G., Bidère N, & Gavard J. (2021). The glycoprotein GP130 governs the surface presentation of the G protein–coupled receptor APLNR. The Journal of Cell Biology, 220(9), e202004114.

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Recombinant Protein Reagents for PD-1 and B7-1 Signaling

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Recombinant proteins human B7–1-hIgG1 and human PD-1-hIgG1 were purchased from R & D systems. Human IgG was purchased from Jackson and BioXcell. Mouse IgG1 isotype control antibody (clone MOPC21) was purchased from BioXcell. Mouse IgG2b and Mouse IgG2a were purchased from Southern Biotech. hPD-1-mIgG2a and hB7–1-mIgG2a were purchased from Chimerigen. hPD-L1-mIgG2a was made in our laboratory (14 (link)).

Chaudhri A., Xiao Y., Klee A.N., Wang X., Zhu B, & Freeman G.J. (2018). PD-L1 binds to B7-1 only in cis on the same cell surface. Cancer immunology research, 6(8), 921-929.

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