Methanol (CH3OH) and ethanol (C2H5OH) were bought from Tianjin Fuyu Fine Chemical Co., ltd. (Tianjin, China). Folin-Ciocialteu reagent (FCR) was acquired from Sangon Biotech Co., ltd. (Shanghai, China). Sodium carbonate (Na2CO3) and ammonium acetate (C2H7NO2) were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China), and hydrogen peroxide (H2O2) was procured from Chron Chemicals (Chengdu, China). 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, C14H18O4), metaphosphoric acid (HPO3), neocuproine (C14H12N2), aluminium chloride (AlCl3), sodium acetate (C2H3NaO2), potassium persulfate (K2S2O8), salicylic acid (C7H₆O3), 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ: C18H12N6), and quercetin (C15H10O7) were supplied by Aladdin Biochemical Technology Co., ltd. (Shanghai, China). Sodium nitrite (NaNO2) was purchased from Lingfeng Chemical Reagent Co., ltd. (Shanghai, China). 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS: C18H18N4O6S4), Ascorbic acid (C6H8O6), 2,6-dichlorophenolindophenol (C12H7NCl2O2), ferrous sulfate (FeSO4), copper dichloride (CuCl2), sodium hydroxide (NaOH), ferric chloride (FeCl3), sodium chloride (NaCl), and gallic acid (C7H6O5) were procured from Shanghai Macklin Biochemical Co., ltd. (Shanghai, China).
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid
Manufactured by Maclin Biochemical Technology
Sourced in China
2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) is a chemical compound commonly used as a substrate in colorimetric enzyme-linked immunosorbent assay (ELISA) and other biochemical assays. It functions as a chromogenic substrate that produces a colored reaction product when oxidized by certain enzymes, allowing for the quantification of the target analyte.
Automatically generated - may contain errors
Lab products found in correlation
2 2 diphenyl 1 picrylhydrazyl (dpph), by Maclin Biochemical Technology (2 mentions)
Hydrogen peroxide h2o2, by Chron Chemicals (1 mentions)
Arbutin, by Maclin Biochemical Technology (1 mentions)
Xylose, by Merck Group (1 mentions)
Monosaccharide standard, by Merck Group (1 mentions)
Mannose, by Merck Group (1 mentions)
Arabinose, by Merck Group (1 mentions)
Rhamnose, by Merck Group (1 mentions)
Galactose, by Merck Group (1 mentions)
2 7 dichlorofluorescein diacetate dcfh da, by Merck Group (1 mentions)
Fructose, by Merck Group (1 mentions)
Cholesterol, by Maclin Biochemical Technology (1 mentions)
4 protocols using 2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid
1
Antioxidant Capacity Measurement Protocols
2
Extraction and Characterization of Peach Flower Polyphenols
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The flowers of Amygdalus persica L. were cultivated in Pingyi County, Linyi City, Shandong Province of China. AB-8 and D101 macroporous resin was obtained from Cangzhou Bon Adsorber Technology Co., Ltd. (Cangzhou, China). 1,1-diphenyl-2-picrylhydrazyl (DPPH), L-Ascorbic acid, 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), arbutin, deoxycholic acid sodium salt, 3,4-Dihydroxy-L-phenylalanine were procured from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Standard monosaccharides (including arabinose, rhamnose, xylose, fructose, mannose, galactose and glucose), lipopolysaccharides (LPS) and 2’,7’-Dichlorofluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All reagents and chemicals were of analytical grade unless stated otherwise. Distilled water was used in all experiments.
3
Antioxidant Potential of Natural Compounds
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TEO was purchased from Florihana (Caussols, France). LYZ (BR, CAS: 12650-88-3) from chicken egg white was purchased from Macklin (Shanghai, China). Phospholipid S100 (S100, 94 % purity) was purchased from Sigma-Aldrich (Milwaukee, USA). Cholesterol (95 % purity, CAS: 57-88-5) was purchased from Macklin (Shanghai, China). 2,2-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS, 98 % purity, CAS: 30931-67-0) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH, 96 % purity, CAS: 1898-66-4) were purchased from Macklin (Shanghai, China). 4,6-Dihydroxy-2-Mercaptopyrimidine (TBA, ≥ 98 % purity, CAS: 504-17-6) and trichloroacetic acid (TCA, 99 % purity, CAS: 76-03-9) were purchased from Adamas (Shanghai, China). All reagents used in this study were analytical pure, and ultra-pure water was used throughout the experimental procedure.
4
Jujube Fermentation with Thermophilic Lactic Acid Bacteria
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Ziziphus jujuba cv. Jinsixiaozao was purchased from Laoling (Shandong Province, China). Streptococcus thermophilus CICC6220 was purchased from the China Center of Industrial Culture Collection (Beijing, China). This strain was in lyophilized form. The jujubes and LAB strain were stored at 4 °C prior to use. Chromatography-grade organic acid standards and phenolics standards were purchased from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China) and Solarbio Science and Technology Co., Ltd (Beijing, China), respectively. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were of 96% purity and were purchased from Macklin (Shanghai, China). All other reagents used were of analytical grade and were purchased from Sinopharm Chemical Reagent Company (Shanghai, China).
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