New bovine calf serum

Manufactured by Thermo Fisher Scientific

Sourced in United States

New bovine calf serum is a cell culture supplement derived from the blood of young bovine calves. It provides a rich source of nutrients, growth factors, and other components necessary for the growth and maintenance of a variety of cell types in vitro.

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Lab products found in correlation

2 protocols using new bovine calf serum

Preparation and Evaluation of Quercetin Derivatives

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quercetin-3-O-rutinoside-7-O-α-l-rhamnosidase (QRR) was prepared in the laboratory [30 (link)]. Procyanidin A₂ and procyanidin B₂ were purchased from ChromaDex (Irvine, CA, USA). Epicatechin, gallic acid, quercetin, vanillin, Folin-Ciocalteu reagent, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), fluorescein sodium, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), and 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH) were obtained from Sigma-Aldrich, Inc. (St. Louis, MO, USA). HPLC-grade glacial acetic acid, acetonitrile, and methanol were obtained from Thermo Fisher Scientific (Suwanee, GA, USA). DMEM(H) medium, new bovine calf serum and Hank’s balanced salt solution (HBSS) were purchased from Gibco Life Technologies (Grand Island, NY, USA). All other reagents were of analytical grade. HepG2 human liver cancer cells were obtained from the American Type Culture Collection (Rockville, MD, USA).

Deng M., Deng Y., Dong L., Ma Y., Liu L., Huang F., Wei Z., Zhang Y., Zhang M, & Zhang R. (2018). Effect of Storage Conditions on Phenolic Profiles and Antioxidant Activity of Litchi Pericarp. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(9), 2276.

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Extraction and Characterization of Citrus Bioactives

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Cigranoside A, B, C, D, E, F, bergamjuicin, neoeriocitrin, melitidin, rhoifolin, and naringin were prepared in our laboratory (Deng et al., 2021 (link)). Hesperidin, neoHesperidin, narirutin, isoquercitrin, (+)-catechin, quercetin, gallic acid, Folin–Ciocalteu reagent, DCFH-DA, Trolox, AAPH, fluorescein sodium, 4-methylumbelliferyl oleate (4-MUO), α-glucosidase, α-amylase and pancreatic lipase, and 4-nitrophenyl-α-d-glucopyranoside (pNPG) were obtained from Sigma Aldrich Co. (St. Louis, MO, USA). HBSS, new bovine calf serum and DMEM (H) medium were obtained from Gibco Life Technologies (Grand Island, NY, USA). HepG2 human liver cancer cells were purchased from the ATCC (Rockville, MD, USA). Acetonitrile and glacial acetic acid in HPLC-grade were purchased from Thermo Fisher Scientific (Suwanee, GA, USA).

Deng M., Dong L., Jia X., Huang F., Chi J., Muhammad Z., Ma Q., Zhao D., Zhang M, & Zhang R. (2022). The flavonoid profiles in the pulp of different pomelo (Citrus grandis L. Osbeck) and grapefruit (Citrus paradisi Mcfad) cultivars and their in vitro bioactivity. Food Chemistry: X, 15, 100368.

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