Mouse monoclonal antibody against Flag and anti-Flag M2 affinity gel were purchased from Sigma (St. Louis, MO). Rabbit monoclonal antibody against HA Tag and phospho-Smad2 (Ser465/467) were obtained from Cell Signaling Technology (Danvers, MA). Mouse monoclonal antibody against PGM1 and rabbit polyclonal antibody against FOXJ2 were purchased from Abcam (Cambridge, MA). Flag peptide and mouse monoclonal antibody against Actin were obtained from Abclonal Technology (Wuhan, China). Rabbit polyclonal antibody against CNBP and rabbit polyclonal antibody against GRP78 were obtained from Proteintech Group (Wuhan, China). Mouse monoclonal antibody against p53 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). D-Galactose and Tunicamycin were obtained from MedChemExpress (Monmouth Junction, NJ). TGFβ recombinant protein was bought from Peprotech (New Jersey). Puromycin and hygromycin were bought from EMD Biosciences (SanDiego, CA). In vitro DNA transfection reagent was purchased from Signagen Laboratories (Rockville, MD). GelCode Blue Stain Reagent was obtained from Pierce (Rockford, IL).
Flag peptide
Manufactured by ABclonal
The Flag peptide is a commonly used protein tag that can be attached to recombinant proteins to aid in their detection and purification. It is a short, highly antigenic peptide sequence derived from the Influenza hemagglutinin (HA) protein. The Flag peptide allows for efficient immunoaffinity purification and detection of the tagged protein using specific anti-Flag antibodies.
Automatically generated - may contain errors
Lab products found in correlation
In vitro dna transfection reagent, by SignaGen (2 mentions)
Mouse monoclonal antibody against flag, by Merck Group (1 mentions)
Anti flag m2 affinity gel, by Merck Group (1 mentions)
Tunicamycin, by MedChemExpress (1 mentions)
N acetyl l cysteine a9165, by Merck Group (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Caspase glo 3 7 assay, by Promega (1 mentions)
γ 32p atp, by PerkinElmer (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by BD (1 mentions)
Deadend fluorometric tunel system, by Promega (1 mentions)
2 protocols using flag peptide
1
Antibody and protein sourcing protocol
2
Apoptosis and Oxidative Stress Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Flag peptide was obtained from Abclonal. Trizol was bought from Life Technologies. The Annexin V‐FITC/PI apoptosis detection kit was bought from BD Biosciences. The DeadEnd Fluorometric TUNEL System was bought from Promega. The SOD2 activity Assay Kit was bought from Beyotime Biotechnology. N‐acetyl‐l ‐cysteine (A9165) was bought from Sigma‐Aldrich. PIEZO1 agonist (Yoda1), MEK1/2 inhibitor (PD0325901), and PI3K inhibitor (LY294002) were obtained from Selleckchem. EGTA Acetoxymethyl ester (HY‐D0973) was bought from MedChemExpress. D‐luciferin was bought from Xenogen. Ampicillin, kanamycin, puromycin, and hygromycin were bought from EMD Biosciences. In vitro DNA transfection reagent was purchased from Signagen Laboratories. [γ‐32P] ATP was bought from PerkinElmer. Recombinant full‐length human AKT1 was bought from SignalChem. Caspase‐Glo 3/7 Assay was bought from Promega.
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