The reference standards of 1,3-diaminopropane, putrescine, cadaverine hydrochloride, spermidine hydrochloride, spermine, agmatine sulfate salt, N-acetylputrescine hydrochloride, N-acetylspermine trihydrochloride, N-acetylspermidine dihydrochloride, l -ornithine hydrochloride, lysine, l -arginine, S-adenosyl-l -methionine, γ-aminobutyric acid and 1, 6-diaminohexane (used as an internal standard) were all obtained from Sigma-Aldrich (St. Louis, MO, USA). HPLC grade methanol was purchased from Fisher Chemicals (Fair Lawn, NJ, USA). Heptafluorobutyric acid (HFBA) was obtained from Sigma-Aldrich. All the other reagents were of analytic grade. Redistilled and deionized water was used throughout the study.
N acetylspermine trihydrochloride
Manufactured by Merck Group
Sourced in United States
N-acetylspermine trihydrochloride is a laboratory compound used for research purposes. It is a modified form of the naturally occurring polyamine spermine. The compound serves as a tool for scientific investigations, but its specific applications are not detailed here.
Automatically generated - may contain errors
Lab products found in correlation
Spermine, by Merck Group (4 mentions)
Spermidine hydrochloride, by Merck Group (4 mentions)
Putrescine, by Merck Group (4 mentions)
Cadaverine hydrochloride, by Merck Group (4 mentions)
N acetylputrescine hydrochloride, by Merck Group (4 mentions)
N acetylspermidine dihydrochloride, by Merck Group (4 mentions)
L arginine, by Merck Group (3 mentions)
L ornithine hydrochloride, by Merck Group (3 mentions)
Heptafluorobutyric acid hfba, by Merck Group (3 mentions)
Agmatine sulfate salt, by Merck Group (3 mentions)
1 3 diaminopropane, by Merck Group (2 mentions)
1 6 diaminohexane, by Merck Group (2 mentions)
Deuterated histamine, by Merck Group (2 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
γ aminobutyric acid, by Merck Group (1 mentions)
S adenosyl l methionine, by Merck Group (1 mentions)
Lc ms grade methanol, by Merck Group (1 mentions)
Gemini c18, by Phenomenex (1 mentions)
Methanol, by Merck Group (1 mentions)
Q exactive orbitrap based mass spectrometer, by Thermo Fisher Scientific (1 mentions)
4 protocols using n acetylspermine trihydrochloride
1
Quantitative Analysis of Biogenic Amines
2
Comprehensive Polyamine Metabolite Analysis
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The reference standards of putrescine, cadaverine hydrochloride, spermidine hydrochloride, spermine, agmatine sulfate salt, N-acetyl-putrescine hydrochloride, N-acetyl-spermine trihydrochloride, N-acetyl-spermidine dihydrochloride, L-ornithine hydrochloride, lysine, L-arginine, S-adenosyl-1-methionine, aminobutyric acid, deuterated histamine, heptafluorobutyric acid (HFBA), and methanol LC/MS grade were purchased from Sigma-Aldrich (St. Louis, MO, USA). Water for LCMS was purchased from Fisher Scientific (Fair Lawn, NJ, USA).
3
LC-HRMS Analysis of Polyamines and Derivatives
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The reference standards of putrescine, cadaverine hydrochloride, spermidine hydrochloride, spermine, agmatine sulphate salt, N-acetyl-putrescine hydrochloride, N-acetylspermine trihydrochloride, N-acetylspermidine dihydrochloride, l-ornithine hydrochloride, lysine, l-arginine, aminobutyric acid, deuterated histamine, heptafluorobutyric acid (HFBA) and methanol were purchased from Sigma-Aldrich (St. Louis, MO, USA) for analytical type analysis. Water for LCMS was purchased from Fisher Scientific (Fair Lawn, NJ, USA).
Liquid chromatography–high-resolution mass spectrometry (LC-HRMS) analysis was performed using an UPLC Ultimate 3000 (Thermo Fisher-Dionex San Jose, CA, USA) system equipped with a HESI-II electrospray source to a Q-Exactive-Orbitrap™-based mass spectrometer (all from Thermo Scientific, San Jose, CA, USA). Chromatographic separation was carried out on the C18 column of the Gemini C18 (Phenomenex, Torrance, CA, USA), 100 mm × 2 mm, particle size 3 µm, the column was held at 37 °C. Chromatographic separation was achieved with gradient elution using a mobile phase composed of 0.05% heptafluorobutyric acid (HFBA) in water (A) and 0.05% HFBA in methanol (B).
Liquid chromatography–high-resolution mass spectrometry (LC-HRMS) analysis was performed using an UPLC Ultimate 3000 (Thermo Fisher-Dionex San Jose, CA, USA) system equipped with a HESI-II electrospray source to a Q-Exactive-Orbitrap™-based mass spectrometer (all from Thermo Scientific, San Jose, CA, USA). Chromatographic separation was carried out on the C18 column of the Gemini C18 (Phenomenex, Torrance, CA, USA), 100 mm × 2 mm, particle size 3 µm, the column was held at 37 °C. Chromatographic separation was achieved with gradient elution using a mobile phase composed of 0.05% heptafluorobutyric acid (HFBA) in water (A) and 0.05% HFBA in methanol (B).
4
Quantitative Analysis of Polyamines
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The reference standards -1,3-diaminopropane, putrescine, cadaverine hydrochloride, spermidine hydrochloride, spermine, agmatine sulfate salt, N-acetylputrescine hydrochloride, N-acetylspermine trihydrochloride, Nacetylspermidine dihydrochloride, and 1,6-diaminohexane (used as an internal standard [IS]) -were all obtained from Sigma-Aldrich (St. Louis, MO). N 1 , N 12 -diacetylspermine n-hydrochloride and N 1 , N 8 -diacetylspermidine were obtained from Wako (Osaka, Japan). Methanol of high-performance liquid chromatography (HPLC) grade was purchased from Fisher Chemicals (Fair Lawn, NJ). Perfluoropentanoic acid was obtained from Sigma-Aldrich (St. Louis, MO).
Raw materials of Mylabris, Ginseng Radix et Rhizoma, Astragali Radix, and Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis were purchased from Tianyitang Chinese Drugstore (Shenyang, China). These drugs were identified by a Professor of Pharmacognosy in Shenyang Pharmaceutical University (China). The Aidi injection was prepared according to the Ministerial Standards of Chinese Medicine. All other reagents were of analytical grade. Redistilled and deionized water was used throughout the study.
Raw materials of Mylabris, Ginseng Radix et Rhizoma, Astragali Radix, and Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis were purchased from Tianyitang Chinese Drugstore (Shenyang, China). These drugs were identified by a Professor of Pharmacognosy in Shenyang Pharmaceutical University (China). The Aidi injection was prepared according to the Ministerial Standards of Chinese Medicine. All other reagents were of analytical grade. Redistilled and deionized water was used throughout the study.
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