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Fumonisin b3

Manufactured by Cayman Chemical
Sourced in United States

Fumonisin B3 is a chemical standard used for analytical purposes. It is a mycotoxin produced by certain species of Fusarium fungi. Fumonisin B3 is primarily used as a reference material for the identification and quantification of this compound in various matrices, such as food, feed, and biological samples, through analytical techniques like chromatography and mass spectrometry.

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2 protocols using fumonisin b3

1

Fumonisin B Compound Characterization

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Fumonisin B1 and B2 were purchased from Abcam (Cambridge, MA, USA). Fumonisin B3 was purchased from Cayman (Ann Arbor, MI, USA). Hydrolyzed fumonisin B1 was obtained from Romer (Beijing, China). Ultrapure water (18.2 MΩ·cm) used in our experiments was supplied by Millipore (Bedford, IN, USA). Restriction enzymes, T4 DNA ligase, and plasmid extraction kits were purchased from Takara Biomedical Technology (Beijing, China). Protein markers, protein-loading buffers, and SDS gel stain washing buffers were purchased from Epizyme (Shanghai, China). Methanol, formic acid, and acetonitrile were purchased from Anpel (Shanghai, China). All other reagents and chemicals were of analytical grade.
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2

Cytotoxicity Evaluation of Fumonisin Analogues

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Fumonisin B1 (ab142433) and fumonisin B2 (ab142434) were obtained from Abcam (Cambridge, MA, USA). Fumonisin B3 (20434) was obtained from Cayman (Ann Arbor, MA, USA). Myriocin (476300-5MG) was obtained from Merck/Millipore (Billerica, MA, USA) [9 (link)]. A Cell Counting Kit-8 (CCK-8) (CK04-3000T) was purchased from DOJINDO Laboratories (Kumamoto, Japan). Antibiotic solution (streptomycin, penicillin, and amphotericin) (03-033-1B) and 0.25% trypsin solution (03-050-1B) were purchased from BioInd (Kibbutz Beit, Israel). Resveratrol (R107315) was purchased from Shanghai Aladdin Biochemical Technology (Shanghai, China). The fetal bovine serum (10099141C) was purchased from Invitrogen (Waltham, MA, USA). RPMI Medium 1640 (11835030) was purchased from Gibco (Waltham, MA, USA).
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