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Mla1213

Manufactured by ADInstruments
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Sourced in Australia

The MLA1213 is a data acquisition device designed for use in laboratory environments. It features 12 analog input channels with 13-bit resolution, enabling the collection and recording of various types of sensor data. The device supports a sampling rate of up to 1 kHz per channel and provides connectivity options for further data analysis and processing.

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Lab products found in correlation

Labchart, by ADInstruments (2 mentions) Octal bio amp, by ADInstruments (2 mentions) Powerlab 4 30 system, by ADInstruments (1 mentions) Animal bio amp, by ADInstruments (1 mentions) Mlt0202, by ADInstruments (1 mentions) Pl3516 p, by ADInstruments (1 mentions) Mla0320, by ADInstruments (1 mentions) Fe224, by ADInstruments (1 mentions) Powerlab 8 35, by ADInstruments (1 mentions) Homeothermic monitoring system, by Harvard Apparatus (1 mentions) Powerlab 16sp, by ADInstruments (1 mentions) Mlt0670, by ADInstruments (1 mentions) Quad bio amp, by ADInstruments (1 mentions)

7 protocols using mla1213

1

Cardiac Arrhythmia Assessment in Animal Model

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ECG signals were recorded at 4- and 8-week endpoints. Animals were anesthetized using 5% isoflurane inhalation with 100% oxygen followed by intubation and respiratory support, placed on a controlled heating pad, and then anesthesia was maintained with 2% isoflurane inhalation with 100% oxygen. An ECG signal was recorded for 30 min with an Animal Bio Amp that attached to a PowerLab 4/30 system, by inserting a needle anode (MLA1213, ADInstruments) into the left front leg of the animal, a needle cathode into the right front leg, and using the testis skin as ground. LabChart (ADInstruments) was used for analysis of malicious arrhythmia, categorized as frequent atrial premature beats (APBs), atrial tachycardia (AT), atrial fibrillation, frequent ventricular premature beats (VPBs), ventricular tachycardia, and ventricular fibrillation.
Tao Z.W., Wu S., Cosgriff-Hernandez E.M, & Jacot J.G. (2019). Evaluation of a polyurethane-reinforced hydrogel patch in a rat right ventricle wall replacement model. Acta biomaterialia, 101, 206-218.
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2

Electrical Stimulation and Contractile Force of Tissue Constructs

Cited 1 time
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At the time of tissue construct formation, spontaneous and electrically paced twitch forces (10 V, 0.1 s) were measured within a thermostated water bath (37°C) using a high-sensitivity isometric force transducer (MLT0202, ADInstruments, Colorado Springs, CO, USA) connected to a quad bridge amplifier (FE224, ADInstruments). ECG signals were recorded using a Bio Amp (FE136, ADInstruments) as previously described.39 (link) Data were acquired through a 16-channel PowerLab system (PL3516/P, ADInstruments). The ECG of the tissue construct was detected by inserting a needle cathode (MLA1213, ADInstruments) into the center of the construct and a needle anode in one of the four construct corners. The media immersing the tissue was used as a ground. The contractile twitch force was measured by attaching the force transducer arm to one free-corner of the square construct, while the other three ends of the square construct were held fixed by pins. Pre-tension was adjusted using a micro-manipulator (Radnoti LLC, Monrovia, CA, USA), set at 1000–2000 μN, force during spontaneous contraction was recorded. LabChart (ADInstruments) was used for data analysis. Electrical pacing was performed by attaching stimulating electrodes (MLA0320, ADInstruments) to the edge of the tissue and pacing at frequencies of 0.25 and 1–5 Hz (in 1-unit increments).
Tao Z.W., Mohamed M., Jacot J.G, & Birla R.K. (2018). Bioengineering Cardiac Tissue Constructs with Adult Rat Cardiomyocytes. ASAIO journal (American Society for Artificial Internal Organs : 1992), 64(5), e105-e114.
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3

Comprehensive Murine ECG Acquisition Protocol

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The surface lead II ECG signal was acquired using limb needle electrodes (MLA1213, AD Instruments, Sydney, Australia). The mice were placed in supine position on a homeothermic monitoring system (Harvard Apparatus, Holliston, MA, USA) to maintain the body temperature at 36–37 °C. Prior to the placement of limb electrodes, the mice were anesthetized using 200 mg/kg ketamine and 20 mg/kg xylazine i.p. along with 10 units of heparin. The needle electrodes were positioned under the skin, with the negative electrode on the right front paw, the positive electrode on left front paw, and the reference electrode on the left rear paw. ECG signals were recorded using an animal bioamplifier supplemented with PowerLab 8/35 and analyzed using LabChart Pro V.8 software (AD instruments). The signals were amplified and sampled at a rate of 1 kHz. An average of 1 min of the ECG signal was used in LabChart software to the calculate HR, P duration, PR interval, QRS duration, and QT interval. The QT interval was corrected for the RR interval according to the Mitchell formula: QTc = QT/√(RR × 10) [43 (link)].
Ozhathil L.C., Rougier J.S., Arullampalam P., Essers M.C., Ross-Kaschitza D, & Abriel H. (2021). Deletion of Trpm4 Alters the Function of the Nav1.5 Channel in Murine Cardiac Myocytes. International Journal of Molecular Sciences, 22(7), 3401.
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4

Continuous Monitoring of Vital Signs in Rats

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Arterial blood pressure (BP) was continuously monitored and recorded for all the rats. A catheter placed in the left femoral artery was connected to a blood pressure transducer (MLT0670, ADInstruments, USA) for continuous recording of BP. Needle-type electrodes (MLA1213, ADInstruments, USA) were inserted subcutaneously to the hind and forelimbs bilaterally to continuously record the ECG. A laser Doppler probe to measure CBF was positioned over a 2 mm craniotomized portion of the frontal bone ipsilateral to the site of CCI. All data were digitized at 1 kHz with PowerLab digitizer (PowerLab 16/SP, ADInstruments, USA).
Li C., Shah K.A., Powell K., Wu Y.C., Chaung W., Sonti A.N., White T.G., Doobay M., Yang W.L., Wang P., Becker L.B, & Narayan R.K. (2021). CBF oscillations induced by trigeminal nerve stimulation protect the pericontusional penumbra in traumatic brain injury complicated by hemorrhagic shock. Scientific Reports, 11, 19652.
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5

Optogenetic Cardiac Modulation via AAV6

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Mice injected with AAV6-CAG-DIO-bReaChES-TS-eYFP and recovered as above were anesthetized with isoflurane (3% induction, 1–2% maintenance) and body temperature maintained at 37°C. Single lead ECG was recorded using needle electrodes (ADInstruments MLA1213), an ADInstruments Octal Bio Amp, and an ADInstruments PowerLab data acquisition system. Respiration was simultaneously recorded using a spirometer (ADInstruments). The implanted fiber optic cannula was connected using a fiber optic cable (Thorlabs M77L01) to a 577 nm laser (CNI Laser). Laser light was delivered using the following parameters: 10–15 mW power from the fiber tip, 10 ms pulse width, 40 Hz. To assess the role of muscarinic receptors, atropine (Sigma A0132, dissolved at 50 mg/mL in ethanol, then prepared as a 0.5 mg/mL working solution in PBS) was administered (10 mg/kg, I.P.) and optogenetic stimulation was repeated 20 minutes later. To calculate the percent change in heart rate, the minimum sinus rate (60 divided by the P-P interval in seconds) during stimulation was subtracted from the sinus rate immediately before stimulation and divided by the pre-stimulation sinus rate. To calculate the percent change in P-R interval, the P-R interval immediately before stimulation was subtracted from the maximum P-R interval during stimulation and divided by the pre-stimulation P-R interval.
Veerakumar A., Yung A.R., Liu Y, & Krasnow M.A. (2022). Molecularly defined circuits for cardiovascular and cardiopulmonary control. Nature, 606(7915), 739-746.
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6

Optogenetic Cardiac Modulation via AAV6

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Mice injected with AAV6-CAG-DIO-bReaChES-TS-eYFP and recovered as above were anesthetized with isoflurane (3% induction, 1–2% maintenance) and body temperature maintained at 37°C. Single lead ECG was recorded using needle electrodes (ADInstruments MLA1213), an ADInstruments Octal Bio Amp, and an ADInstruments PowerLab data acquisition system. Respiration was simultaneously recorded using a spirometer (ADInstruments). The implanted fiber optic cannula was connected using a fiber optic cable (Thorlabs M77L01) to a 577 nm laser (CNI Laser). Laser light was delivered using the following parameters: 10–15 mW power from the fiber tip, 10 ms pulse width, 40 Hz. To assess the role of muscarinic receptors, atropine (Sigma A0132, dissolved at 50 mg/mL in ethanol, then prepared as a 0.5 mg/mL working solution in PBS) was administered (10 mg/kg, I.P.) and optogenetic stimulation was repeated 20 minutes later. To calculate the percent change in heart rate, the minimum sinus rate (60 divided by the P-P interval in seconds) during stimulation was subtracted from the sinus rate immediately before stimulation and divided by the pre-stimulation sinus rate. To calculate the percent change in P-R interval, the P-R interval immediately before stimulation was subtracted from the maximum P-R interval during stimulation and divided by the pre-stimulation P-R interval.
Veerakumar A., Yung A.R., Liu Y, & Krasnow M.A. (2022). Molecularly defined circuits for cardiovascular and cardiopulmonary control. Nature, 606(7915), 739-746.
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7

Diaphragm EMG Electrode Placement

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The skin of the xiphoid process at the lower end of the animal’s sternum was incised, and a small incision of about 2 cm was cut along the linea alba to open the abdominal cavity.
Needle electrodes (MLA1213, ADInstruments, Sydney, Australia) inserted into the exposed diaphragm were connected to the Biological Amplifier (FE234, Quad Bio Amp, ADInstruments, Sydney, Australia) for collection of bilateral EMGdi signals (left EMGdi and right EMGdi).
Gu X., Guo Z., Cai M., Shi Y., Wang S, & Xie F. (2023). Paced breathing and respiratory movement responses evoked by bidirectional constant current stimulation in anesthetized rabbits. Frontiers in Bioengineering and Biotechnology, 10, 1109892.
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