Tissue culture reagents were obtained from Lonza (Basel, Switzerland, www.lonza.com ) unless otherwise stated and fetal bovine serum (FBS) from Gibco (Paisley, UK, www. thermofisher.com/uk/en/home/brands/gibco.html ). Biochemical reagents were obtained from Sigma‐Aldrich (St. Louis, MO, www.sigmaaldrich.com ) unless otherwise stated.
Tissue culture reagents
Manufactured by Lonza
Sourced in United Kingdom
Tissue culture reagents are a suite of products designed to support the growth and maintenance of cell cultures. These reagents include media, supplements, and other essential components required for culturing a variety of cell types in a controlled laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
Biochemical reagents, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trifast, by Avantor (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
Epidermal growth factor (egf), by Merck Group (1 mentions)
Ly294002, by Cell Signaling Technology (1 mentions)
Sds page reagents, by Bio-Rad (1 mentions)
Fibronectin, by Roche (1 mentions)
Phospho akt s473, by Cell Signaling Technology (1 mentions)
Sugen 5416, by Cayman Chemical (1 mentions)
Mouse antihuman β actin monoclonal antibody, by Abcam (1 mentions)
Pd98059, by Cell Signaling Technology (1 mentions)
First strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Phospho p38 mapk thr180 tyr182, by Cell Signaling Technology (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Taqman gene expression assay, by Thermo Fisher Scientific (1 mentions)
Sb203580, by Cell Signaling Technology (1 mentions)
Gelatin, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Endothelial cell growth supplement (ecgs), by PromoCell (1 mentions)
5 protocols using tissue culture reagents
1
Cell Culture Reagent Procurement
2
Steroidogenesis Pathway Protein Analysis
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ApoA-I and HDL were purchased from Athens Research and Technology (Georgia, U.S.A.). All other materials were from U.K. companies or U.K. suppliers for the companies indicated. Radiochemicals were purchased from PerkinElmer, Tri-Fast from PeqLab and cDNA synthesis kits from Bioline. Rabbit monoclonal antibodies to Cyp27A1, ADX and LXR, and polyclonal antibodies to TSPO, ADXR and ABCA1 were purchased from Abcam; rabbit polyclonal antibody to StarD1 was from Santa Cruz Biotechnology. Sodium pentobarbital was supplied by J.M. Loveridge PLC (Southampton), tissue culture reagents from Lonza and sterile tissue culture plastics from Greiner. The clones for Cyp27A1, ADX and ADXR (pCMV) were from Origene.com via Cambridge Biosciences. Complete™ protease inhibitor cocktail was purchased from Roche, and chemicals, TLC plates and solvents from Sigma–Aldrich.
3
Quantifying IGFBP-1 Signaling Pathways
4
VEGF-Induced Signaling Pathway Analysis
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Tissue culture reagents, unless otherwise specified, were obtained from Biowhittaker (Walkersville, MD). Human recombinant VEGF, bFGF and epidermal growth factor (EGF) were purchased from PrepoTech (Rocky Hill, NJ). Sugen 5416 was obtained from Cayman Chemical Company (Ann Arbor, MI) and human VEGF neutralizing antibody was purchased from R&D Systems Europe (Abingdon, UK). Inhibitors LY294002, PD98059, and SB203580 were purchased from Cell Signaling Technology, Inc. (Bioke, Leiden, NL). Antibodies against Akt, Phospho-Akt (S473), p44/42 MAPK (ERK1/2), Phospho-p44/42 MAPK (ERK1/2), p38 MAPK, and Phospho- p38 MAPK (Thr180/Tyr182) were from Cell Signaling and mouse anti-human β actin monoclonal antibody was from Abcam (Cambridge, UK). Gelatin was obtained from Sigma-Aldrich and Fibronectin from Roche Diagnostics. SDS-PAGE reagents were obtained from BioRad (Hercules, CA). TaqMan Gene Expression assays were purchased from Applied Biosystems (Carlsbad, CA), TRIzol reagent and First Strand cDNA synthesis kits were purchased from Invitrogen (Carlsbad, CA). All other reagents were from Sigma-Aldrich Chemie B.V. (Zwijndrecht, NL), unless stated otherwise.
5
Chick Embryo Tissue Culture
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Fertilized, wild-type D1, Bovans Brown chick eggs were obtained from Henry Stewart & Co. (Norfolk, United Kingdom). Tissue culture reagents were purchased from Lonza (Nottingham, United Kingdom). Fetal calf serum (FCS) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Endothelial cell growth supplement (ECGS) was purchased from Promocell (Heidelberg, Germany). Tissue culture plastics were obtained from Corning (Barry, United Kingdom). Cell culture plate inserts were procured from Thermo Fisher Scientific (Millicell-CM, 0.4 µm pore size, 30 mm diameter). Histologic reagents and materials were purchased from Thermo Fisher Scientific.
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