Sepharose cl 6b

Sepharose CL-2B, Sepharose CL-4B, and Sepharose CL-6B resins (Cytiva) were washed with PBS in a glass bottle. The volume of resin was washed three times with an equal volume of PBS before use. Econo-Pac Chromatography columns (Bio-Rad) were packed with resin and a frit was inserted into the column above the resin. For all columns in Figures 4 and 5, each column was washed with 10 ml PBS (twice 5 ml at a time) prior to loading of sample. For SEC columns, resin was added until the bed volume (resin without liquid) reached 10 ml. For DMC columns, Fractogel EMD SO3- (M) (MilliporeSigma) was added as a bottom layer with 2 ml bed volume, and 10 ml of Sepharose CL-6B bed volume was added as a top layer. For TMC columns, a 2:1 by volume (of dry resin) mixture was prepared of Fractogel EMD SO3- (M) (MilliporeSigma) and Capto Core 700 (Cytiva) and 2 ml bed volume bottom layer was added to the column before 10 ml of Sepharose CL-6B bed volume was added as a top layer.