Following transcardiac perfusion with ice-cold PBS, spinal cords of mice were harvested and homogenized using a lysis buffer containing protease inhibitor cocktail and phosphatase inhibitor. Levels of proinflammatory cytokines including interleukin-1β (IL-1β; RayBiotech, USA), interleukin-6 (IL-6; RayBiotech, USA) and tumor necrosis factor-α (TNF-α; RayBiotech, USA), and growth factors including brain-derived neurotrophic factor (BDNF; MyBioSource, USA), glial cell line-derived neurotrophic factor (GDNF; MyBioSource, USA) and vascular endothelial growth factor (VEGF; MyBioSource, USA) in the homogenates were determined using mouse ELISA kits according to the manufacturer’s instructions.
Vascular endothelial growth factor (vegf)
Manufactured by MyBioSource
Sourced in United States
VEGF (Vascular Endothelial Growth Factor) is a protein that plays a crucial role in the formation and regulation of blood vessels. It is responsible for stimulating the growth and proliferation of endothelial cells, which are the cells that line the interior of blood vessels. VEGF is essential for angiogenesis, the process of new blood vessel formation, and is involved in various physiological and pathological processes.
Automatically generated - may contain errors
Lab products found in correlation
Brain derived neurotrophic factor (bdnf), by MyBioSource (1 mentions)
Mouse elisa kit, by MyBioSource (1 mentions)
Interleukin 6 (il 6), by RayBiotech (1 mentions)
Tnf α, by RayBiotech (1 mentions)
Il 1β, by RayBiotech (1 mentions)
Bcl 2, by Novus Biologicals (1 mentions)
Interleukin 6 (il 6), by Abcam (1 mentions)
Tgf β1, by MyBioSource (1 mentions)
Tgf β, by MyBioSource (1 mentions)
Mbs175904, by MyBioSource (1 mentions)
Ethanol, by Merck Group (1 mentions)
Interleukin 6 (il 6), by MyBioSource (1 mentions)
Il 10, by MyBioSource (1 mentions)
Tnf α, by MyBioSource (1 mentions)
4 protocols using vascular endothelial growth factor (vegf)
1
Cytokine and Growth Factor Analysis
2
Quantifying Intestinal Inflammation Markers
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The following ELISA kits and their sources (in parentheses) were used to assess the colon contents of NF-κB (Novus Biologicals, CO, USA; Cat # NBP2- 29661), Bcl-2 (Novus Biologicals; Cat# NBP2-69947), E-cadherin (PicoKine ELISA Kit; Boster Biological Technology, CA, USA; Cat# EK1434), VEGF (MyBioSource; Cat # MBS724516), IL-6 (Biovision, CA, USA; Cat # K4145-100), GSK3-β (Aviva Systems Biology, CA, USA; Cat # OKEH03023) and pS473-Akt (DRG, NJ, USA; Cat # EIA-3997) following the manufacturers, protocols.
3
Biomarker Assessment in Biological Samples
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The corresponding ELISA kits were used to assess MDA (LifeSpan Biosciences; WA; USA; Cat.# LS-F28018), SOD (MyBioSource; CA, USA; Cat.# MBS036924), PAF (MyBioSource; CA, USA; Cat.# MBS2024376), TGF-β1 (MyBioSource; CA, USA; Cat.# MBS011634), HIF-1α (Kamia Biomedical; Seattle, USA; Cat.# KT-17920) and VEGF (MyBioSource; CA, USA; Cat.# MBS8506132). All steps were carried out in accordance with the manufacturers’ instructions. For the determination of protein content, the method of Lowry et al. (1951 (link)) was performed.
4
Bioactive Compound Extraction and Quantification
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Ethanol was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Normal saline (0.9 % w/v) was prepared from the Samen® pharmacy factory, Iran. Xylazine, acepromazine, and ketamine were prepared from Alfasen, Woerden, Holland. Also, enzyme-linked immunosorbent assay (ELISA) kits, including VEGF, IL-6, TGF-β, IL-10, and TNF-α, were obtained from MyBioSource, USA with catalog number respectively; MBS3015758, MBS8244673, MBS2701296, MBS2707969, MBS175904. Dichloromethane (DCM) was purchased from Caledon, Canada, for HPLC grade. In addition, deionized water and Ethanol (96 %) were obtained from Abtin Co. (Iran), Kian Kaveh Azma Co. (Iran), and Taghtir Khorasan Co. (Iran), respectively. Furthermore, 6-gingerol was purchased from Golexir Pars ® Co., Iran, as an internal standard. Other chemicals and reagents were from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).
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