Blood samples were drawn after an overnight fast (12 hours). Blood pressure was measured by an automatic upper arm monitor (Omron M6; OMRON Healthcare Europe, Hoofddorp, the Netherlands). Blood lipids, glucose, insulin, and C‐reactive protein were measured by standard laboratory methods at Uppsala University Hospital. Homeostasis model assessment of insulin resistance was calculated as glucose×insulin/22.5. ELISA was used to determine proinsulin (Mercodia, Uppsala, Sweden), PCSK9 (CycLex, Nagano, Japan), FGF21, IL‐6, IL‐8, IL‐15, IL‐1 receptor antagonist, TNFα, vascular cell adhesion molecule‐1, intercellular adhesion molecule‐1, E‐selectin, endostatin, and TNF receptor 2 (R&D Systems, Minneapolis, MN). Serum unesterified lathosterol was determined by isotope dilution mass spectrometry and corrected for total cholesterol.7 (link) Total NEFA were analyzed using the NEFA FS response kit (Cat. No. 157819910921; DiaSys Diagnostic Systems, Holzheim, Germany), an enzymatic end point method automatically measured on Response 910 (DiaSys Diagnostic Systems) according to the manufacturer's instructions. Von Willebrand factor was measured by ELISA with antisera from Dako (Glostrup, Denmark). The assay was calibrated against Liatest (DiagnosticaStago, Asnieres, France).
Vascular cell adhesion molecule 1
Manufactured by R&D Systems
Sourced in Japan
Vascular cell adhesion molecule-1 is a cell surface sialoglycoprotein that mediates the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. It is a member of the immunoglobulin superfamily and plays a role in leukocyte-endothelial cell adhesion and signal transduction.
Automatically generated - may contain errors
Lab products found in correlation
E selectin, by R&D Systems (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Proinsulin, by Mercodia (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Endostatin, by R&D Systems (1 mentions)
Il 1 receptor antagonist, by R&D Systems (1 mentions)
Liatest, by Diagnostica Stago (1 mentions)
Il 15, by R&D Systems (1 mentions)
Accucheck counting beads, by Thermo Fisher Scientific (1 mentions)
Sdf1a, by R&D Systems (1 mentions)
Ccl21, by R&D Systems (1 mentions)
2 protocols using vascular cell adhesion molecule 1
1
Metabolic and Inflammatory Biomarkers Assessment
2
Transwell Migration and Cell Adhesion Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For Transwell migration assays, stromal cell-derived factor 1 (SDF1a; R&D Systems, Minneapolis, Minn) and CCL21 (R&D Systems) were added to the lower chamber, and 200,000 cells were placed in the upper chamber. Cells were collected in the lower chamber after 4 hours at 378C. Migrated cells were counted (by using AccuCheck Counting Beads, Life Technologies) and analyzed by means of flow cytometry. All experiments were performed in triplicate. For the cell adhesion assays, wells were coated with retronectin-CH 296 (Takara, Shiga, Japan) or vascular cell adhesion molecule 1 (R&D Systems), and 200,000 cells were incubated for 1 hour at 378C. Adherent cells were collected (after washing with PBS with 2 mmol/L EDTA) and counted. For the detection of the high-affinity lymphocyte function-associated antigen 1 (LFA-1), cells were cultured in the presence of 10 mmol/L EDTA (the nonactivated condition) or 1 mmol/L MgCl 2 (the activated condition) plus SDF1a, as previously described. 26 After 5 minutes of activation, cells were stained with the A579 ligand mimetic antibody (kindly provided by T. A. Springer, Harvard Medical School, Boston, Mass) and analyzed by means of flow cytometry.
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