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Chemiluminescent immunoassay kit

Manufactured by Roche
Sourced in Germany

The Chemiluminescent Immunoassay Kit is a laboratory equipment used for the detection and quantification of specific analytes in a sample. It utilizes a chemiluminescent reaction to generate a measurable signal proportional to the amount of target analyte present in the sample.

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4 protocols using chemiluminescent immunoassay kit

1

Venous Blood Biomarker Assessment

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Blood was taken from an antecubital vein in the morning, sober and after staying extended for 20 min. Blood samples were collected into tubes containing potassium ethylenediaminetetraacetic acid (EDTA) (1 g/L plasma) and N-terminal proBNP were calculated with the Cobas Elecsys E411 analyzer (range 5–35,000 pg/mL) using chemiluminescent immunoassay kit (Roche Diagnostics, Grenach -Wyhlen, Germany).
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2

Fasting Blood N-terminal proBNP Measurement

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Fasting venous blood was collected from study participants after they had rested in a supine position for 20 min. Samples were placed in disposable EDTA containers (1 g/L of plasma), and N-terminal proBNP was measured by a Cobas Elecsys E 411 analyzer (measuring range 5–35000 pg/mL) using a chemiluminescent immunoassay kit (Roche Diagnostics, Grenach-Wyhlen, Germany).
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3

Baicalin Modulates Granulosa Cell Estradiol

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The primary granulosa cells were seeded onto 6-well plates at a density of 1 × 106 cells per well, conventionally cultured for 24 h, starved in the starvation culture medium for 24 h, and then treated with the corresponding concentrations of Baicalin with 10− 7 M testosterone (Solarbio) added simultaneously to the medium as the substrate for estradiol (E2) production. After 48 h of treatment, the cell culture supernatant was collected, and the concentrations of estradiol and progesterone were analyzed using a chemiluminescent immunoassay kit (Roche Diagnostics, Rotkreuz, Switzerland) and cobas 6000 analyzer series (Roche Diagnostics) according to the kit manufacturer’s instructions.
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4

Venous Blood Biomarkers in Admission

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Venous blood was collected on the admission and NT-pro-BNP was measured using a chemiluminescent immunoassay kit (Roche Diagnostics, Grenzach-Wyhlen, Germany), with a normal range of 0–125 pg/mL. Routine blood indices and serum creatinine were also tested. We calculated estimated glomerular filtration rate (eGFR) using the Chinese version of the four-variable Modification of Diet in Renal Disease equation. The demographic and clinical characteristics of study participants were collected via an electronic case report by one researcher and were randomly checked by another one. Transthoracic motion-mode, two-dimensional and Doppler echocardiographic evaluations were routinely performed within 24 hours of admission. Left ventricular ejection fraction (LVEF) was evaluated through the Simpson’s biplane method.
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