Cells were harvested 48 h after transfection, and lysates were prepared. Immunoblotting was carried out with rabbit anti‐SKA1 antibodies (1:1,000 dilution, SAB2701430; Sigma‐Aldrich), anti‐AKT antibody (1:1000, #4691; Cell Signaling Technology, Danvers, MA, USA), anti‐p‐AKT antibody (1:1000, #4060; Cell Signaling Technology), anti‐ERK1/2 antibody (1:1000, #4695; Cell Signaling Technology), anti‐p‐ERK1/2 antibody (1:2000, #4370; Cell Signaling Technology), anti‐FAK antibody (1:1000, #3285; Cell Signaling Technology) and anti‐p‐FAK antibody (1:1000, #8556; Cell Signaling Technology), anti‐SRC antibody (1:1000, #2123; Cell Signaling Technology) and anti‐p‐SRC antibody (1:1000, #6943; Cell Signaling Technology). Anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) antibodies (1:10000, ab8245; Abcam, Cambridge, UK) were used as an internal loading control. Experimental procedures were carried out as described previously.22 , 25 , 26 , 27 , 28 Protein expression was quantified by using NIH‐ImageJ.
Sab2701430
Manufactured by Merck Group
Sourced in United States
SAB2701430 is a laboratory equipment product manufactured by Merck Group. This product is designed for laboratory use, however, a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. As a result, further details on the intended use of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
Anti erk1 2 antibody, by Cell Signaling Technology (1 mentions)
Anti src antibody, by Cell Signaling Technology (1 mentions)
Anti p erk1 2 antibody, by Cell Signaling Technology (1 mentions)
Anti fak antibody, by Cell Signaling Technology (1 mentions)
Anti p fak antibody, by Cell Signaling Technology (1 mentions)
Anti akt antibody, by Cell Signaling Technology (1 mentions)
Anti p src antibody, by Cell Signaling Technology (1 mentions)
Anti p akt antibody, by Cell Signaling Technology (1 mentions)
Histofine sab po kit, by Nichirei Biosciences (1 mentions)
Ab175951, by Abcam (1 mentions)
Ab8245, by Abcam (1 mentions)
3 protocols using sab2701430
1
Immunoblotting Analysis of Key Signaling Proteins
2
Immunohistochemical Analysis of SKA1 Protein
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Tissue specimens were incubated overnight at 4°C with anti‐SKA1 antibodies (1:500 dilution, SAB2701430; Sigma‐Aldrich). The slides were treated with biotinylated goat antibodies (Histofine SAB‐PO kit; Nichirei, Tokyo, Japan). The procedures were described previously.22 , 25 , 26 , 27 , 28
3
Immunoblotting Analysis of SKA1 and SKA3
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Cells were harvested 48 h after transfection, and lysates were prepared. Immunoblotting was performed with anti-SKA1 antibodies (1:500 dilution, SAB2701430; Sigma-Aldrich, St. Louis, MO, USA) and anti-SKA3 antibodies (1:1,000 dilution, ab175951; Abcam, Cambridge, UK). Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies (1:10,000 dilution, ab8245; Abcam) were used as an internal loading control. The procedures were described previously [29 (link), 42 (link), 43 (link)].
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