Lsm 5 pascal head

Two microscopes were used in the experiments: A LEO 912AB transmission electron microscope (Zeiss, Jena, Germany) equipped with Quantax 200 for energy-dispersive X-ray spectroscopy (EDS) and a Zeiss 510 META confocal microscope (Zeiss, Jena, Germany) coupled with Axiovert 200 M. The inverted microscope Axiovert 200 M is equipped with an LSM 5 Pascal head (with magnification 200×), which facilitates non-invasive visualization of such structures as biofilms, microcapsules, and other film-forming substances as well as crystallization of substances by means of laser fluorescence, standard fluorescence, and polarization contrast. Moreover, it allows geometric and densitometric measurements as well as assessment of the structure edge. In addition, the experiments were conducted with the use of a CT Skyscan 1172 tomograph (Brucker MicroCT, Kontich, Belgium).

The 200 μL of Fluorescence Brightener 28 (F3543-1G, Sigma Aldrich, Steniheim, Germany) fluorescent dye was added to 1 mg of the EPS precipitates and incubated in the dark for 25 min. After this time, the dye solution was separated from the EPS by centrifugation, and then the pellet was applied to a slide and observed with excitation at 365 nm and emission at 435 nm under an Axivert 200M confocal microscope (Carl Zeiss Microscopy GmbH, Jena, Germany) equipped with a Zeiss LSM5 Pascal head