Nitazoxanide (99%, CAS: 55981‐09‐4) was purchased from Shanghai Yuanye Biotechnology Co., Ltd. and diluted into a stock solution of 10 mg/ml with dimethyl sulfoxide (DMSO) and stored at room temperature. The working solution was prepared by diluting the stock solution with embryo culture solution (0.3325 g/L sea salt, 4.2 mM sodium bicarbonate; pH = 7.2) before conducting the experiment. Kits for measuring enzyme activity or other biological indicators such as malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and ROS were purchased from Nanjing Jiancheng Institute of Biological Engineering (Nanjing, China). RNA extraction kits, cDNA reverse transcriptase kits and Quantitative real‐time PCR (qPCR) reagents were purchased from Takara (Japan). TUNEL Apoptosis Detection Kit (Alexa Fluor 640) was purchased from Shanghai yeasen Biotechnology Co., Ltd. Gadofullerene nanoparticles (GFNPs) were kindly granted by the Institute of Chemistry, Chinese Academy of Sciences, Beijing, China. All other biochemical reagents were purchased from Sangon Biotech (Shanghai, China) and were analytically pure.
Tunel apoptosis detection kit alexa fluor 640
Manufactured by Yeasen
Sourced in China
The TUNEL Apoptosis Detection Kit (Alexa Fluor 640) is a laboratory equipment used to detect and analyze apoptosis, a form of programmed cell death, in biological samples. The kit utilizes the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) assay, which labels the free 3'-OH DNA ends generated during apoptosis with Alexa Fluor 640 dye. This allows for the visualization and quantification of apoptotic cells using fluorescence microscopy or flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Yuanye Bio-Technology (1 mentions)
Rna extraction kit, by Takara Bio (1 mentions)
Malondialdehyde, by Nanjing Jiancheng (1 mentions)
Fluorescence microscope, by Olympus (1 mentions)
Real envision detection system, by Agilent Technologies (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Anti bcl 2, by Affinity Biosciences (1 mentions)
Anti bax, by Affinity Biosciences (1 mentions)
Ecl plus kit, by PerkinElmer (1 mentions)
Anti mtor, by Affinity Biosciences (1 mentions)
Qpcr reagents, by Takara Bio (1 mentions)
Bicinchoninic acid bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Anti sqstm1 p62, by Affinity Biosciences (1 mentions)
Anti p ampk, by Affinity Biosciences (1 mentions)
Anti ampk, by Affinity Biosciences (1 mentions)
Anti p53, by Affinity Biosciences (1 mentions)
Anti p mtor, by Affinity Biosciences (1 mentions)
β actin, by Beyotime (1 mentions)
Proliferating cell nuclear antigen (pcna), by Abmart (1 mentions)
7 protocols using tunel apoptosis detection kit alexa fluor 640
1
Nitazoxanide Antioxidant and Apoptosis Effects
2
Apoptosis Detection via TUNEL Assay
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Apoptosis was detected with TUNEL Apoptosis Detection Kit (Alexa Fluor 640; YEASEN). Cells were inoculated into an 18‐mm cover glass coverslip in a 12‐well plate and fixed by pre‐cold acetic acid/ethanol reagent. Afterwards, they were washed with PBS and dyed with TUNEL reagent. Sections were mounted, and fluorescence images were captured.
3
Apoptosis Detection in Paraffin Sections
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Paraffin-embedded tissue sections were stained with the TUNEL Apoptosis Detection Kit (Alexa Fluor 640) (Yeasen, Shanghai, China) according to the manufacturer’s protocol. Images were acquired with an Olympus fluorescence microscope (FV1000; Olympus, Tokyo, Japan).
4
Apoptosis Detection in Paraffin Sections
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Paraffin-embedded tissue sections were stained with the TUNEL Apoptosis Detection Kit (Alexa Fluor 640) (Yeasen, Shanghai, China) according to the manufacturer's protocol. Images were acquired with an Olympus fluorescence microscope (Olympus, Tokyo, Japan).
5
Shikonin Analysis and RNA Extraction
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Shikonin analysis reference substance was purchased from Beijing Soleibao Technology Co., Ltd., Beijing, China. RNA extraction kit Tranzol UP (ET111–01), cDNA Transcription Kit (AE311–03) and TransStart@ Tip Green qPCR Supermix (AQ601–02) were purchased from Beijing Quanjin Biotechnology Co., Ltd. Reactive oxygen detection kits were purchased from Nanjing Jiancheng Institute of Bioengineering (Nanjing, China). TUNEL Apoptosis Detection Kit (Alexa Fluor 640) was purchased from Yeasen Company (Shanghai, China).
6
Femoral Vein Apoptosis and PI3K/AKT Signaling
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The femoral vein tissue was dehydrated in graded concentrations of ethanol and embedded in paraffin. The femoral vein tissue was cut into sections with a thickness of 4 µm. Following dewaxing and rehydration, cell apoptosis of the femoral vein tissue was examined using a TUNEL Apoptosis Detection kit (Alexa Fluor 640; Shanghai Yeasen Biotechnology Co., Ltd.) according to the manufacturer's instructions. The femoral vein tissue was stained with 2 µg/ml DAPI (Beyotime Institute of Biotechnology) for 5 min at 25°C in a dark room. Sections of the femoral vein tissue were also incubated with anti-PI3K (1:100), anti-p-PI3K (1:100), anti-AKT (1:100), anti-p-AKT (1:100) and anti-factor XII (1:100) antibodies overnight at 4°C, followed by incubation with HRP-conjugated secondary antibodies (1:50) for 45 min at 37°C. After the final wash, the sections were observed using a Dako REAL EnVision Detection system.
7
Fibroblast growth factor 21 modulates oxidative stress
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Fibroblast growth factor 21 standard protein was provided by Wenzhou Medical University (Wenzhou, China); metronidazole was purchased from Shenggong (Shanghai) Biotechnology Co., Ltd.; chloroquine (HY-17589A) was purchased from MCE Biotechnology Co., Ltd.; and glutathione (GSH), malondiazole aldehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) detection kits were purchased from Nanjing Jiancheng Institute of Bioengineering (Nanjing, China). Bicinchoninic acid (BCA) protein assay kits were purchased from Thermo Fisher Scientific (Rockford, Illinois, United States), and the ECL Plus kit was purchased from PerkinElmer Life Sciences (Waltham, MA, United States). The TUNEL apoptosis detection kit (Alexa Fluor 640) was purchased from Yeasen Biotechnology Co., Ltd. (Shanghai, China), and qPCR reagents were purchased from Takara (Dalian, China).
The following primary antibodies were purchased: PCNA and LC3B from Abmart Inc. (Shanghai, China); Anti-p53, Anti-bcl2, Anti-bax, Anti-AMPK, Anti-p-AMPK, Anti-mTOR, Anti-p-mTOR, and Anti-SQSTM1/p62 from Affinity Biosciences (Cincinnati, OH, United States); and 4’,6-diamidino-2-phenylindole (DAPI), protein loading buffer, and β-actin from Beyotime Biotechnology (Jiangsu, China).
The following primary antibodies were purchased: PCNA and LC3B from Abmart Inc. (Shanghai, China); Anti-p53, Anti-bcl2, Anti-bax, Anti-AMPK, Anti-p-AMPK, Anti-mTOR, Anti-p-mTOR, and Anti-SQSTM1/p62 from Affinity Biosciences (Cincinnati, OH, United States); and 4’,6-diamidino-2-phenylindole (DAPI), protein loading buffer, and β-actin from Beyotime Biotechnology (Jiangsu, China).
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