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Deadend tunel staining kit

Manufactured by Promega
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The DEADEnd TUNEL staining kit is a laboratory product that provides a method for detecting and quantifying apoptosis, or programmed cell death, in cells. The kit utilizes the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) assay to label the fragmented DNA that is characteristic of apoptotic cells. This allows for the identification and analysis of cells undergoing apoptosis.

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TUNEL kit (56 citations) TUNEL staining (24 citations) TUNEL staining kit (16 citations)

2 protocols using deadend tunel staining kit

1

TUNEL Staining for Apoptosis Quantification

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TUNEL staining was conducted using the DEADEnd TUNEL staining kit (Promega Cat# G3250). Sections were deparaffinized in a graded ethanol series, re-fixed in 10% Formalin, and permeabilized with 20 ug/ml of Proteinase K. All other steps were followed according to the manufacturer’s instructions. Sections were counterstained with DAPI (Vector Laboratories, Inc, Burlingame, CA Cat # H-1200). Mesenchymal and ectodermal TUNEL positive cells were quantified independently.
Belus M.T., Rogers M.A., Elzubeir A., Josey M., Rose S., Andreeva V., Yelick P.C, & Bates E.A. (2018). Kir2.1 is important for efficient BMP signaling in mammalian face development. Developmental biology, 444(Suppl 1), S297-S307.
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2

Apoptotic Cell Detection Using TUNEL Assay

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Apoptotic cells were assessed by a DeadEnd TUNEL-staining kit (Promega), as previously described (23) .
Tomida C., Yamagishi N., Aibara K., Yano C., Uchida T., Abe T., Ohno A., Hirasaka K., Nikawa T, & Teshima-Kondo S. (2015). Chronic exposure of VEGF inhibitors promotes the malignant phenotype of colorectal cancer cells. The journal of medical investigation : JMI, 62(1-2).
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