w1118 or genetic background strains were used as control lines in this study. We used previously generated mutant and transgenic lines: UAS-TnT (Deitcher et al., 1998 (link); Sweeney et al., 1995 (link)); Chats2 and Chats3 alleles (Salvaterra and McCaman, 1985 (link)); UAS-Dα7-EGFP (Leiss et al., 2009 (link)) and Dα7PΔEY6 (Fayyazuddin et al., 2006 (link)); UAS-NACHO-3xHA (Bischof et al., 2013 (link)); UAS-mLexA-VP16-NFAT, LexAop-CD8-GFP, and LexAop-CD2-GFP transgenes (Masuyama et al., 2012 (link)); NFATΔAB, NFATΔA, and NFATΔB alleles (Keyser et al., 2007 (link)); elav-GAL4 (elav-GAL4c155) and tub-GAL80ts (Bloomington Drosophila Stock Center, Indiana University); 201Y-GAL4 (O’Dell et al., 1995 (link); Yang et al., 1995 (link)); GH146-GAL4 (Stocker et al., 1997 (link)); UAS-RNAi-NACHO (VDRC, Vienna, Austria), Dα7-GAL4 (Fayyazuddin et al., 2006 (link)). All Drosophila stocks were raised at 18–25ºC as specified in text, on standard fly food medium.
Elav gal4 elav gal4c155 and tub gal80ts
Manufactured by Bloomington Drosophila Stock Center
Elav-GAL4 (elav-GAL4c155) is a Drosophila melanogaster driver line that expresses the GAL4 transcription factor under the control of the elav neuronal promoter. The tub-GAL80ts is a temperature-sensitive GAL80 repressor that inhibits GAL4 activity at lower temperatures.
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2 protocols using elav gal4 elav gal4c155 and tub gal80ts
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Genetic Manipulation in Drosophila
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Genetic Manipulation in Drosophila
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w1118 or genetic background strains were used as control lines in this study. We used previously generated mutant and transgenic lines: UAS-TnT (Deitcher et al., 1998 (link); Sweeney et al., 1995 (link)); Chats2 and Chats3 alleles (Salvaterra and McCaman, 1985 (link)); UAS-Dα7-EGFP (Leiss et al., 2009 (link)) and Dα7PΔEY6 (Fayyazuddin et al., 2006 (link)); UAS-NACHO-3xHA (Bischof et al., 2013 (link)); UAS-mLexA-VP16-NFAT, LexAop-CD8-GFP, and LexAop-CD2-GFP transgenes (Masuyama et al., 2012 (link)); NFATΔAB, NFATΔA, and NFATΔB alleles (Keyser et al., 2007 (link)); elav-GAL4 (elav-GAL4c155) and tub-GAL80ts (Bloomington Drosophila Stock Center, Indiana University); 201Y-GAL4 (O’Dell et al., 1995 (link); Yang et al., 1995 (link)); GH146-GAL4 (Stocker et al., 1997 (link)); UAS-RNAi-NACHO (VDRC, Vienna, Austria), Dα7-GAL4 (Fayyazuddin et al., 2006 (link)). All Drosophila stocks were raised at 18–25ºC as specified in text, on standard fly food medium.
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